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使用化学蛋白质组学方法鉴定抗癌天然产物的靶点

Target identification of anticancer natural products using a chemical proteomics approach.

作者信息

Bhukta Swadhapriya, Gopinath Pushparathinam, Dandela Rambabu

机构信息

Department of Industrial and Engineering Chemistry, Institute of Chemical Technology Indianoil Odisha Campus, Samantpuri Bhubaneswar 751013 India

Department of Chemistry, SRM-Institute of Science and Technology Kattankulathur 603203 Chennai Tamilnadu India.

出版信息

RSC Adv. 2021 Aug 18;11(45):27950-27964. doi: 10.1039/d1ra04283a. eCollection 2021 Aug 16.

DOI:10.1039/d1ra04283a
PMID:35480761
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9038044/
Abstract

In recent years, there has been a strong demand worldwide for the identification and development of potential anticancer drugs based on natural products. Natural products have been explored for their diverse biological and therapeutic applications from ancient time. In order to enhance the efficacy and selectivity and to minimize the undesired side effects of anti cancer natural products (ANPs), it is essential to understand their target proteins and their mechanistic pathway. Chemical proteomics is one of the most powerful tools to connect ANP target identification and quantification where labeling and non-labeling based approaches have been used. Herein, we have discussed the various strategies to systemically develop selective ANP based chemical probes to characterise their specific and non-specific target proteins using a chemical proteomic approach in various cancer cell lysates.

摘要

近年来，全球对基于天然产物的潜在抗癌药物的鉴定和开发有着强烈的需求。从古代起，人们就探索了天然产物的多种生物学和治疗应用。为了提高抗癌天然产物（ANPs）的疗效和选择性，并尽量减少其不良副作用，了解它们的靶蛋白及其作用机制至关重要。化学蛋白质组学是连接ANP靶点鉴定和定量的最强大工具之一，其中已使用了基于标记和非标记的方法。在此，我们讨论了各种策略，以系统地开发基于选择性ANP的化学探针，使用化学蛋白质组学方法在各种癌细胞裂解物中表征其特异性和非特异性靶蛋白。

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