Chatterjee Satadru, Meador William E, Smith Cameron, Chandrasiri Indika, Zia Mohammad Farid, Nguyen Jay, Dorris Austin, Flynt Alex, Watkins Davita L, Hammer Nathan I, Delcamp Jared H
Department of Chemistry and Biochemistry, University of Mississippi, Coulter Hall, University MS 38677 USA
Department of Biological Sciences, University of Southern Mississippi Hattiesburg MS 39406 USA.
RSC Adv. 2021 Aug 16;11(45):27832-27836. doi: 10.1039/d1ra05479a.
Shortwave infrared (SWIR) emission has great potential for deep-tissue biological imaging with high resolution. In this article, the synthesis and characterization of two new xanthene-based RosIndolizine dyes coded RosIndz and RosIndz is presented. The dyes are characterized femtosecond transient absorption spectroscopy as well as steady-state absorption and emission spectroscopies. The emission of these dyes is shown in the SWIR region with peak emission at 1097 nm. RosIndz was encapsulated with an amphiphilic linear dendritic block co-polymer (LDBC) coded 10-PhPCL-G3 with high uptake yield. Further, cellular toxicity was examined using HEK (human embryonic kidney) cells where a >90% cell viability was observed at practical concentrations of the encapsulated dye which indicates low toxicity and reasonable biocompatibility.
短波红外（SWIR）发射在高分辨率深部组织生物成像方面具有巨大潜力。本文介绍了两种新的基于呫吨的玫瑰吲哚嗪染料RosIndz和RosIndz的合成与表征。这些染料通过飞秒瞬态吸收光谱以及稳态吸收和发射光谱进行表征。这些染料在SWIR区域发射，峰值发射波长为1097nm。RosIndz被一种编码为10-PhPCL-G3的两亲性线性树枝状嵌段共聚物（LDBC）包裹，摄取产率高。此外，使用HEK（人胚胎肾）细胞检测了细胞毒性，在包裹染料的实际浓度下观察到细胞活力>90%，这表明其毒性低且生物相容性良好。
