检测霍乱肠毒素的免疫荧光法
[Immunofluorescence method of detecting cholera enterotoxin].
作者信息
Vladimtseva I V, Efremenko V I, Pushkar' V G, Goloseev Iu A, Trofimov E N
出版信息
Zh Mikrobiol Epidemiol Immunobiol. 1986 Dec(12):62-5.
PMID:3548172
Abstract
The quantitative immunofluorescent assay for the determination of cholera enterotoxin is proposed. The assay is based on the selective sorption of cholera enterotoxin by gangliosides incorporated into polyacrylamide granules. The preliminary treatment of gangliosides with neuraminidase enhances the sensitivity of this assay. The assay permits the detection of cholerigen in an amount of 20 ng.
摘要
本文提出了一种用于测定霍乱肠毒素的定量免疫荧光测定法。该测定法基于结合到聚丙烯酰胺颗粒中的神经节苷脂对霍乱肠毒素的选择性吸附。用神经氨酸酶对神经节苷脂进行预处理可提高该测定法的灵敏度。该测定法能够检测到20纳克的霍乱原。
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