Trofimov E N, Efremenko V I, Narbutovich N I, Pushkar' V G
Zh Mikrobiol Epidemiol Immunobiol. 1987 Feb(2):57-60.
A variant of EIA techniques for the determination of cholera enterotoxin is proposed. This method is based on the selective sorption of the toxin on ganglioside-containing magnetic granules with its subsequent detection by means of immune serum and antispecific immunoperoxidase conjugate. The proposed method permits the detection of 0.052 +/- 0.02 ng of protein of the purified toxin.
提出了一种用于测定霍乱肠毒素的酶免疫分析(EIA)技术变体。该方法基于毒素在含神经节苷脂的磁性颗粒上的选择性吸附,随后通过免疫血清和抗特异性免疫过氧化物酶结合物进行检测。所提出的方法能够检测出0.052±0.02纳克纯化毒素的蛋白质。