Maestre-Batlle Danay, Nygaard Unni C, Huff Ryan D, Alexis Neil E, Tebbutt Scott J, Turvey Stuart E, Carlsten Christopher, Kocbach Bølling Anette
Department of Medicine, Air Pollution Exposure Lab and Legacy for Airway Health, University of British Columbia and Vancouver Coastal Health, Vancouver, Canada.
Department of Environmental Health, Norwegian Institute of Public Health, Oslo, Norway.
Indoor Air. 2022 Apr;32(4):e13026. doi: 10.1111/ina.13026.
Phthalates are ubiquitous environmental contaminants associated with allergic disease in epidemiological and animal studies. This investigation aims to support these associations by interrogating systemic immune effects in allergen-sensitized volunteers after controlled indoor air exposure to a known concentration of dibutyl phthalate (DBP). The phthalate-allergen immune response (PAIR) study enrolled 16 allergen-sensitized participants to a double-blinded, randomized, crossover exposure to two conditions (DBP or control air for 3 hr), each followed immediately by inhaled allergen challenge. Peripheral blood immune cell composition and activation along with inflammatory mediators were measured before and after exposure. DBP exposure prior to the inhaled allergen challenge increased the percentage of CD4 T helper cells and decreased the percentage of regulatory T cells (3 hr and 20 hr post-exposure), while only modest overall effects were observed for inflammatory mediators. The cells and mediators affected by the phthalate exposure were generally not overlapping with the endpoints affected by allergen inhalation alone. Thus, in distinction to our previously published effects on lung function, DBP appears to alter endpoints in peripheral blood that are not necessarily enhanced by allergen alone. Further studies are needed to clarify the role of phthalate-induced systemic effects in disease pathogenesis.
邻苯二甲酸盐是普遍存在的环境污染物，在流行病学和动物研究中与过敏性疾病相关。本研究旨在通过在已知浓度的邻苯二甲酸二丁酯（DBP）室内空气受控暴露后，探究变应原致敏志愿者的全身免疫效应，来支持这些关联。邻苯二甲酸盐-变应原免疫反应（PAIR）研究招募了16名变应原致敏参与者，进行双盲、随机、交叉暴露于两种条件（DBP或对照空气3小时），每种条件后立即进行吸入变应原激发试验。在暴露前后测量外周血免疫细胞组成和活化以及炎症介质。在吸入变应原激发试验前暴露于DBP会增加CD4辅助性T细胞百分比并降低调节性T细胞百分比（暴露后3小时和20小时），而炎症介质仅观察到适度的总体效应。受邻苯二甲酸盐暴露影响的细胞和介质通常与仅受变应原吸入影响的终点不重叠。因此，与我们之前发表的对肺功能的影响不同，DBP似乎会改变外周血中的终点，而这些终点不一定仅由变应原增强。需要进一步研究来阐明邻苯二甲酸盐诱导的全身效应在疾病发病机制中的作用。
