Teng Kai Wen, Koide Akiko, Koide Shohei
Perlmutter Cancer Center, New York University Langone Medical Center, New York, NY, USA.
Discovery Biologics, Merck & Co., Inc., Boston, MA, USA.
Methods Mol Biol. 2022;2491:143-154. doi: 10.1007/978-1-0716-2285-8_8.
Molecular display technologies have enabled the generation of synthetic binders with high affinities against a variety of antigens. However, engineering binders with high selectivity is still a challenging task. Here, we illustrate points to consider in developing highly selective binders against antigens of interest. We describe a systematic strategy for sorting selective binders using the yeast display technology. Using the approach described, our group has overcome molecular recognition challenges and developed a series of synthetic binders with exceptional selectivity against diverse antigens.
分子展示技术已能够生成对多种抗原具有高亲和力的合成结合物。然而，设计具有高选择性的结合物仍然是一项具有挑战性的任务。在此，我们阐述了在开发针对目标抗原的高选择性结合物时需要考虑的要点。我们描述了一种使用酵母展示技术筛选选择性结合物的系统策略。通过所描述的方法，我们团队克服了分子识别方面的挑战，并开发出了一系列对多种抗原具有卓越选择性的合成结合物。
