State Key Laboratory of Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai 200092, China.
Shanghai Municipal Engineering Design Institute (Group) Co. LTD, 901 Zhongshan North Second Rd, Shanghai 200092, China.
Sci Total Environ. 2022 Aug 20;835:155559. doi: 10.1016/j.scitotenv.2022.155559. Epub 2022 Apr 26.
Hydrogen oxidizing bacteria (HOB), a type of chemoautotroph, are a group of bacteria from different genera that share the ability to oxidize H and fix CO to provide energy and synthesize cellular material. Recently, HOB have received growing attention due to their potential for CO capture and waste recovery. This review provides a comprehensive overview of the biological characteristics of HOB and their application in resource recovery and pollutant removal. Firstly, the enzymes, genes and corresponding regulation systems responsible for the key metabolic processes of HOB are discussed in detail. Then, the enrichment and cultivation methods including the coupled water splitting-biosynthetic system cultivation, mixed cultivation and two-stage cultivation strategies for HOB are summarized, which is the critical prerequisite for their application. On the basis, recent advances of HOB application in the recovery of high-value products and the removal of pollutants are presented. Finally, the key points for future investigation are proposed that more attention should be paid to the main limitations in the large-scale industrial application of HOB, including the mass transfer rate of the gases, the safety of the production processes and products, and the commercial value of the products.
产氢菌（HOB）是一类化能自养细菌，属于不同属的细菌，它们具有氧化 H 和固定 CO 的能力，为其提供能量并合成细胞物质。由于其在 CO 捕集和废物回收方面的潜力，HOB 最近受到了越来越多的关注。本综述全面概述了 HOB 的生物学特性及其在资源回收和污染物去除方面的应用。首先，详细讨论了 HOB 关键代谢过程所涉及的酶、基因和相应的调控系统。然后，总结了 HOB 的富集和培养方法，包括耦合水分解-生物合成系统培养、混合培养和两段式培养策略，这是其应用的关键前提。在此基础上，介绍了 HOB 在回收高价值产品和去除污染物方面的最新进展。最后，提出了未来研究的重点，即应更加关注 HOB 大规模工业应用中的主要限制因素，包括气体的传质速率、生产过程和产品的安全性以及产品的商业价值。
