Centre of Wastewater Resource Recovery, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China; CAS Key Laboratory for Urban Pollutant Conversion, Department of Applied Chemistry, University of Science and Technology of China, Hefei, 230026, China.
Centre of Wastewater Resource Recovery, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.
Chemosphere. 2021 Mar;266:128932. doi: 10.1016/j.chemosphere.2020.128932. Epub 2020 Nov 10.
Biofertilizers can replace chemical fertilizers to promote the plant growth without causing any pollution. The study of hydrogen-oxidizing bacteria (HOB) enrichment as biofertilizers from mixed culture is scarce. Our recent study shows that biofertilizing HOB are successfully enriched in a short hydraulic retention time of 10 h. While, the mechanism is unknown. This study intentionally used a two-stage method to enrich biofertilizing HOB specifically with nitrate as nitrogen source in Stage 1 and then 1-aminocyclopropane-1-carboxylate (ACC) as nitrogen source in Stage 2. It was found Pseudomonas (34.46%, reported HOB) predominated in Stage 1, while Azospirillum (59.35%), Azoarcus (36%) were dominant genera and Azospirillum lipoferum strain DSM 1691 (50%), Azoarcus olearius strain DQS-4 (50%) were dominant species in Stage 2. The enriched HOB of Stage 2 showed ACC deaminase activity. Furthermore, they could also fix N and consume Ca(PO). Thus, the two-stage method can be used as a specific enrichment for HOB as biofertilizers, which extends the application of HOB in agriculture.
生物肥料可以替代化肥，在促进植物生长的同时不会造成任何污染。从混合培养物中富集产氢菌（HOB）作为生物肥料的研究还很少。我们最近的研究表明，在水力停留时间短至 10 小时的情况下，可以成功地从混合培养物中富集产氢菌。然而，其机制尚不清楚。本研究有意采用两段式方法，在第一阶段以硝酸盐作为氮源，专门富集具有生物肥料功能的产氢菌，然后在第二阶段以 1-氨基环丙烷-1-羧酸（ACC）作为氮源。结果发现，在第一阶段，假单胞菌（34.46%，报道的产氢菌）占优势，而在第二阶段，优势属为固氮螺菌（59.35%）、节杆菌（36%），优势种为施氏假单胞菌 DSM 1691（50%）和脱氮副球菌 DQS-4（50%）。第二阶段富集的产氢菌具有 ACC 脱氨酶活性。此外，它们还可以固定氮并消耗 Ca（PO）。因此，两段式方法可作为产氢菌作为生物肥料的特异性富集方法，扩展了产氢菌在农业中的应用。
