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利用微生物联合体从食物垃圾中生产乳酸:重点关注工艺放大的关键参数和发酵残余物的增值。

Lactic acid production from food waste using a microbial consortium: Focus on key parameters for process upscaling and fermentation residues valorization.

机构信息

Suez, CIRSEE, 38 rue du Président Wilson, 78230 Le Pecq, France.

LBE, INRAE, Univ Montpellier, 102 Avenue des Etangs, Narbonne F-11100, France.

出版信息

Bioresour Technol. 2022 Jun;354:127230. doi: 10.1016/j.biortech.2022.127230. Epub 2022 Apr 25.

Abstract

In this study, the production of lactic acid from food waste in industrially relevant conditions was investigated. Laboratory assays were first performed in batch conditions to determine the suitable operational parameters for an efficient lactic acid production. The use of compost as inoculum, the regulation of temperature at 35 °C and pH at 5 enhanced the development of Lactobacillus sp. resulting in the production of 70 g/L of lactic acid with a selectivity of 89% over the other carboxylic acids. Those parameters were then applied at pilot scale in successive fed-batch fermentations. The subsequent high concentration (68 g/L), yield (0.38 g/gTS) and selectivity (77%) in lactic acid demonstrated the applicability of the process. To integrate the process into a complete value chain, fermentation residues were then converted into biogas through anaerobic digestion. Lastly, the experiment was successfully replicated using commercial and municipal waste collected in France.

摘要

本研究旨在工业相关条件下,从食物垃圾中生产乳酸。首先在分批条件下进行实验室试验,以确定高效生产乳酸的合适操作参数。使用堆肥作为接种物,将温度调节至 35°C,pH 值调节至 5,促进了 Lactobacillus sp. 的发展,从而生产出 70g/L 的乳酸,对其他羧酸的选择性为 89%。然后,将这些参数应用于连续分批发酵的中试规模。随后,乳酸的高浓度(68g/L)、产率(0.38g/gTS)和选择性(77%)表明该工艺具有适用性。为了将该工艺整合到完整的价值链中,然后通过厌氧消化将发酵残渣转化为沼气。最后,使用在法国收集的商业和市政废物成功复制了该实验。

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