Leininger Aaron, Lu Sidan, Jiang Jinyue, Bian Yanhong, May Harold D, Ren Zhiyong Jason
Department of Civil and Environmental Engineering, Princeton University, USA.
Andlinger Center for Energy and the Environment, Princeton University, USA.
Environ Sci Ecotechnol. 2024 Jul 27;22:100459. doi: 10.1016/j.ese.2024.100459. eCollection 2024 Nov.
Regulating electron transfer in predominantly fermentative microbiomes has broad implications in environmental, chemical, food, and medical fields. Here we demonstrate electrochemical control in fermenting food waste, digestate, and wastewater to improve lactic acid production. We hypothesize that applying anodic potential will expedite and direct fermentation towards lactic acid. Continued operation that introduced epi/endophytic communities () to pure culture reactors with static electrodes was associated with the loss of anode-induced process intensification despite 80% retention. Employing fluidized electrodes discouraged biofilm formation and extended electrode influence to planktonic gram-positive fermenters using mediated extracellular electron transfer. While short-term experiments differentially enriched and spp., longer-term operations indicated convergent microbiomes and product spectra. These results highlight a functional resilience of environmental fermentative microbiomes to perturbations in redox potential, underscoring the need to better understand electrode induced polymicrobial interactions and physiological impacts to engineer tunable open-culture or synthetic consortia.
在以发酵为主的微生物群落中调节电子传递在环境、化学、食品和医学领域具有广泛的意义。在此，我们展示了在发酵食物垃圾、沼渣和废水中的电化学控制，以提高乳酸产量。我们假设施加阳极电位将加速并引导发酵朝着乳酸方向进行。尽管保留率达80%，但将表生/内生群落引入带有静态电极的纯培养反应器的持续操作与阳极诱导的过程强化的丧失有关。采用流化电极可抑制生物膜形成，并通过介导的细胞外电子传递将电极影响扩展至浮游革兰氏阳性发酵菌。虽然短期实验差异性地富集了特定物种，但长期操作表明微生物群落和产物谱趋同。这些结果突出了环境发酵微生物群落对氧化还原电位扰动的功能恢复力，强调了更好地理解电极诱导的多微生物相互作用和生理影响以设计可调节的开放培养或合成菌群的必要性。
