Guangzhou Eighth People's Hospital, Guangzhou Medical University, 8 Huaying Road, Baiyun District, Guangzhou, 510440, China.
Guangdong Center for Diagnosis and Treatment of AIDS, 627 Dongfeng East Road, Yuexiu District, Guangzhou, 510060, China.
J Antimicrob Chemother. 2022 Jun 29;77(7):1960-1968. doi: 10.1093/jac/dkac132.
To comprehensively analyse the prevalence of drug resistance and the transmission characteristics of CRF59_01B strains in infected patients in Guangdong, China.
CRF59_01B-infected individuals were recruited, and the HIV-1 pol region was amplified. Drug resistance-associated mutations (DRMs) and antiretroviral susceptibility were examined using the Stanford University HIV Drug Resistance Database to analyse pretreatment drug resistance (PDR) and acquired drug resistance (ADR). Genetic transmission networks were extracted from the maximum likelihood phylogenetic tree with Cluster Picker and visualized with Cytoscape.
Two hundred and twenty-five CRF59_01B-infected individuals, comprising 35 ART-experienced and 190 ART-naive individuals, were recruited. No patients harboured PI DRMs, 5.33% (12/225) of the patients harboured NRTI DRMs and 11.11% (25/225) of the patients harboured NNRTI DRMs. The overall prevalence of strains with ADR was 51.43% (18/35), while the prevalence of strains with PDR was 2.63% (5/190). A total of 20 transmission networks, involving 25.78% (58/225) database-derived sequences, were identified. The networks ranged in size from 2 to 10 individuals, of which most (55.00%, 11/20) were made up of two individuals. Among the 225 study subjects, 9.78% (22/225) had 1 link and 16.00% (36/225) had ≥2 links.
The overall prevalence of CRF59_01B strains with ADR among the ART-experienced patients was high. Although the overall prevalence of CRF59_01B strains with PDR among the ART-naive patients was low, it is necessary to remain vigilant regarding some important DRMs.
全面分析中国广东地区感染 CRF59_01B 株的患者中耐药流行率和传播特征。
招募感染 CRF59_01B 的个体，并扩增 HIV-1 pol 区。使用斯坦福大学 HIV 耐药性数据库检测耐药相关突变（DRMs）和抗逆转录病毒药物敏感性，分析预处理耐药（PDR）和获得性耐药（ADR）。从最大似然系统进化树中提取遗传传播网络，使用 Cluster Picker 提取并使用 Cytoscape 可视化。
共招募了 225 名 CRF59_01B 感染者，包括 35 名接受 ART 治疗的患者和 190 名未接受 ART 治疗的患者。没有患者携带 PI DRMs，5.33%（12/225）的患者携带 NRTI DRMs，11.11%（25/225）的患者携带 NNRTI DRMs。总的 ADR 株发生率为 51.43%（18/35），而 PDR 株发生率为 2.63%（5/190）。共鉴定出 20 个传播网络，涉及 25.78%（58/225）数据库衍生序列。网络大小从 2 到 10 个个体不等，其中大多数（55.00%，11/20）由两个个体组成。在 225 名研究对象中，9.78%（22/225）有 1 个传播链，16.00%（36/225）有≥2 个传播链。
接受 ART 治疗的患者中 CRF59_01B 株的 ADR 总体发生率较高。尽管未接受 ART 治疗的患者中 CRF59_01B 株的 PDR 总体发生率较低，但仍需警惕一些重要的 DRMs。
