[中国6省19市男男性行为HIV-1感染者的HIV-1基因亚型及治疗前耐药性分析]
[Analysis of HIV-1 genetic subtype and pretreatment drug resistance among men who have sex with men infected with HIV-1 from 19 cities of 6 provinces in China].
作者信息
Zhang R, Dong T L, Liang W L, Cao Z B, Xie Z, Liu K M, Yu F, Fu G F, Zhang Y Q, Wang G Y, Ma Q Q, Wu S B, Li Y, Dong W, Jiang Z, Xu J, Wu Z Y, Yao J, Pan P L, Qiu M F
机构信息
National HIV Reference Laboratory, National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Division of HIV Prevention and Intervention, National Center for AIDS/STD Control and Prevention,Chinese Center for Disease Control and Prevention, Beijing 102206,China.
出版信息
Zhonghua Liu Xing Bing Xue Za Zhi. 2022 Apr 10;43(4):523-527. doi: 10.3760/cma.j.cn112338-20211125-00918.
To investigate the distribution of HIV-1 genetic subtypes and pretreatment drug resistance (PDR) among men who have sex with men (MSM) from 19 cities of 6 provinces in China. From April to November 2019, 574 plasma samples of ART-naive HIV-1 infected MSM were collected from 19 cities in Hebei, Shandong, Jiangsu, Zhejiang, Fujian, and Guangdong provinces, total ribonucleic acid (RNA) was extracted and amplified the HIV-1 gene region by nested polymerase chain reaction (PCR) after reverse transcription. Then sequences were used to construct a phylogenetic tree to determine genetic subtypes and submitted to the Stanford drug resistance database for drug resistance analysis. A total of 479 samples were successfully amplified by PCR. The HIV-1 genetic subtypes included CRF01_AE, CRF07_BC, B, CRF55_01B, CRF59_01B, CRF65_cpx, CRF103_01B, CRF67_01B, CRF68_01B and unrecognized subtype, which accounted for 43.4%, 36.3%, 6.3%, 5.9%, 0.8%, 0.8%, 0.4%, 0.4%, 0.2% and 5.5%, respectively. The distribution of genetic subtypes among provinces is statistically different (=44.141, <0.001). The overall PDR rate was 4.6% (22/479), the drug resistance rate of non-nucleoside reverse transcriptase inhibitors, nucleoside reverse transcriptase inhibitors, and protease inhibitors were 3.5% (17/479), 0.8% (4/479) and 0.2% (1/479), respectively. The PDR rate of recent infections was significantly higher than that of long-term infections (=4.634, =0.031). The HIV-1 genetic subtypes among MSM infected with HIV-1 from 19 cities of 6 provinces in China are diverse, and the distribution of subtypes is different among provinces. The overall PDR rate is low, while the PDR rate of recent infections was significantly higher than that of long-term infections, suggesting the surveillance of PDR in recent infections should be strengthened.
调查中国6省19个城市男男性行为者（MSM）中HIV-1基因亚型分布及治疗前耐药情况。2019年4月至11月，从河北、山东、江苏、浙江、福建和广东省的19个城市收集了574份未接受抗逆转录病毒治疗（ART）的HIV-1感染MSM的血浆样本，提取总核糖核酸（RNA），逆转录后通过巢式聚合酶链反应（PCR）扩增HIV-1基因区域。然后用序列构建系统发育树以确定基因亚型，并提交至斯坦福耐药数据库进行耐药性分析。共479份样本通过PCR成功扩增。HIV-1基因亚型包括CRF01_AE、CRF07_BC、B、CRF55_01B、CRF59_01B、CRF65_cpx、CRF103_01B、CRF67_01B、CRF68_01B和未识别亚型，分别占43.4%、36.3%、6.3%、5.9%、0.8%、0.8%、0.4%、0.4%、0.2%和5.5%。各省间基因亚型分布存在统计学差异（=44.141，<0.001）。总体治疗前耐药率为4.6%（22/479），非核苷类逆转录酶抑制剂、核苷类逆转录酶抑制剂和蛋白酶抑制剂的耐药率分别为3.5%（17/479）、0.8%（4/479）和0.2%（1/479）。近期感染的治疗前耐药率显著高于长期感染（=4.634，=0.031）。中国6省19个城市感染HIV-1的MSM中HIV-1基因亚型多样，各省间亚型分布不同。总体治疗前耐药率较低，而近期感染的治疗前耐药率显著高于长期感染，提示应加强对近期感染治疗前耐药情况的监测。
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