Chua B H, Russo L A, Gordon E E, Kleinhans B J, Morgan H E
Am J Physiol. 1987 Mar;252(3 Pt 1):C323-7. doi: 10.1152/ajpcell.1987.252.3.C323.
An increase in aortic pressure from 60 to 120 mmHg accelerated ribosomal protein synthesis in rat hearts during 1 or 2 h of labeling with 0.4 mM [3H]phenylalanine. When hearts were perfused with buffer that contained 20 mM glucose and normal plasma concentrations of 19 other amino acids without added insulin, ribosomal protein synthesis relative to the rate of total protein synthesis increased from approximately 0.22 to 0.36 and 0.30 as aortic pressure was raised from 60 to 120 mmHg during 1 or 2 h of labeling, respectively. With the addition of insulin, the relative rate of ribosomal protein synthesis averaged 0.33 at an aortic pressure of 60 mmHg and increased to 0.42 when aortic pressure was raised to 120 mmHg. These results indicate that elevation of aortic pressure has a preferential effect on synthesis of new ribosomes. This response appears to be an early and physiologically significant event in cardiac hypertrophy.
在用0.4 mM [3H]苯丙氨酸标记1或2小时期间，大鼠心脏中主动脉压力从60 mmHg升高到120 mmHg会加速核糖体蛋白合成。当心脏用含有20 mM葡萄糖和19种其他氨基酸正常血浆浓度但未添加胰岛素的缓冲液灌注时，在标记1或2小时期间，随着主动脉压力从60 mmHg升高到120 mmHg，相对于总蛋白合成速率的核糖体蛋白合成分别从约0.22增加到0.36和0.30。添加胰岛素后，在主动脉压力为60 mmHg时，核糖体蛋白合成的相对速率平均为0.33，当主动脉压力升高到120 mmHg时增加到0.42。这些结果表明，主动脉压力升高对新核糖体的合成具有优先作用。这种反应似乎是心脏肥大早期且具有生理意义的事件。
