Canvida Detection Organization, CP 33212, Gijon, Spain.
Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), Hospital Universitario Central de Asturias, Av. de Roma s/n, 33011, Oviedo, Spain.
Sci Rep. 2022 Apr 28;12(1):6911. doi: 10.1038/s41598-022-11013-1.
Sarcomas are mesenchymal cancers which often show an aggressive behavior and patient survival largely depends on an early detection. In last years, much attention has been given to the fact that cancer patients release specific odorous volatile organic compounds (VOCs) that can be efficiently detected by properly trained sniffer dogs. Here, we have evaluated for the first time the ability of sniffer dogs (n = 2) to detect osteosarcoma cell cultures and patient samples. One of the two dogs was successfully trained to discriminate osteosarcoma patient-derived primary cells from mesenchymal stem/stromal cells (MSCs) obtained from healthy individuals. After the training phase, the dog was able to detect osteosarcoma specific odor cues in a different panel of 6 osteosarcoma cell lines with sensitivity and specificity rates between 95 and 100%. Moreover, the same VOCs were also detected by the sniffer dog in saliva samples from osteosarcoma patients (n = 2) and discriminated from samples from healthy individuals with a similar efficacy. Altogether, these results indicate that there are common odor profiles shared by cultures of osteosarcoma cells and body fluid samples from patients and provide a first proof of concept about the potential of canine odor detection as a non-invasive screening method to detect osteosarcomas.
肉瘤是间充质来源的癌症，通常表现出侵袭性的行为，患者的生存很大程度上取决于早期发现。近年来，人们越来越关注这样一个事实，即癌症患者会释放出特定的有气味的挥发性有机化合物（VOCs），这些化合物可以通过经过适当训练的嗅探犬有效地检测到。在这里，我们首次评估了嗅探犬（n=2）检测骨肉瘤细胞培养物和患者样本的能力。其中一只狗成功地接受了训练，可以区分骨肉瘤患者来源的原代细胞与来自健康个体的间充质干细胞（MSCs）。在训练阶段之后，这只狗能够以 95%到 100%的灵敏度和特异性率，从不同的 6 种骨肉瘤细胞系中检测到骨肉瘤特定的气味线索。此外，嗅探犬还在 2 名骨肉瘤患者的唾液样本中检测到了相同的 VOCs，并具有相似的功效将其与健康个体的样本区分开来。总之，这些结果表明，骨肉瘤细胞培养物和患者体液样本之间存在共同的气味特征，并为犬类气味检测作为一种非侵入性筛查方法来检测骨肉瘤的潜在可能性提供了初步的概念验证。
