Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi, China.
Sugarcane Research Center, Chinese Academy of Agricultural Sciences, Nanning, Guangxi, China.
BMC Plant Biol. 2022 Apr 29;22(1):222. doi: 10.1186/s12870-022-03588-8.
Ratoon sugarcane is susceptible to chlorosis, characterized by chlorophyll loss, poor growth, and a multitude of nutritional deficiency mainly occurring at young stage. Chlorosis would significantly reduce the cane production. The molecular mechanism underlying this phenomenon remains unknown. We analyzed the transcriptome and metabolome of chlorotic and non-chlorotic sugarcane leaves of the same age from the same field to gain molecular insights into this phenomenon.
The agronomic traits, such as plant height and the number of leaf, stalk node, and tillers declined in chlorotic sugarcane. Chlorotic leaves had substantially lower chlorophyll content than green leaves. A total of 11,776 differentially expressed genes (DEGs) were discovered in transcriptome analysis. In the KEGG enriched chlorophyll metabolism pathway, sixteen DEGs were found, eleven of which were down-regulated. Two photosynthesis pathways were also enriched with 32 genes downregulated and four genes up-regulated. Among the 81 enriched GO biological processes, there were four categories related to metal ion homeostasis and three related to metal ion transport. Approximately 400 metabolites were identified in metabolome analysis. The thirteen differentially expressed metabolites (DEMs) were all found down-regulated. The phenylpropanoid biosynthesis pathway was enriched in DEGs and DEMs, indicating a potentially vital role for phenylpropanoids in chlorosis.
Chlorophyll production, metal ion metabolism, photosynthesis, and some metabolites in the phenylpropanoid biosynthesis pathway were considerably altered in chlorotic ratoon sugarcane leaves. Our finding revealed the relation between chlorosis and these pathways, which will help expand our mechanistic understanding of ratoon sugarcane chlorosis.
宿根蔗容易出现黄化现象，其特征是叶绿素丧失、生长不良和多种营养缺乏，主要发生在幼叶期。黄化会显著降低蔗产量。其发生的分子机制尚不清楚。我们分析了来自同一田间同一年龄的黄化和非黄化蔗叶片的转录组和代谢组，以期深入了解这一现象。
黄化蔗的农艺性状，如株高和叶片数、茎节数和分蘖数均下降。黄化叶片的叶绿素含量明显低于绿叶。在转录组分析中发现了 11776 个差异表达基因（DEGs）。在 KEGG 富集的叶绿素代谢途径中，发现了 16 个 DEGs，其中 11 个下调。两个光合作用途径也被富集，有 32 个基因下调和 4 个基因上调。在 81 个富集的 GO 生物过程中，有四个与金属离子稳态相关的类别和三个与金属离子转运相关的类别。在代谢组分析中鉴定出约 400 种代谢物。13 个差异表达代谢物（DEMs）均下调。苯丙烷生物合成途径在 DEGs 和 DEMs 中均被富集，表明苯丙烷类物质在黄化中可能具有重要作用。
宿根蔗黄化叶片中叶绿素合成、金属离子代谢、光合作用和苯丙烷生物合成途径中的一些代谢物发生了显著改变。我们的发现揭示了黄化与这些途径之间的关系，有助于我们深入了解宿根蔗黄化的机制。
