Nuriding Hailiqiguli, Wang Xuemei, Shen Yiping, Liu Yu, Yan Mei
Department of the First Internal Medicine, Pediatric Center of the First Affiliated Hospital of Xinjiang Medical University, Urumqi, 830054, Xinjiang, PR China.
Department of Laboratory Medicine, Children's Hospital Boston, Boston, 02115, Massachusetts, United States.
J Biomed Nanotechnol. 2022 Feb 1;18(2):527-534. doi: 10.1166/jbn.2022.3220.
This study aimed to investigate the role of the primary Fos-related antigen 1 (-1) oncogene in nephroblastoma by studying 60 childhood nephroblastoma and 58 paraneoplastic carcinoma cases. The expression was detected using immunohistochemistry. culture of nephroblastoma cells was performed by viral transfection to establish overexpression and gene knockout models. Flow cytometry and nano-PCR were used to detect apoptosis and mRNA expression in related pathway genes. Immunohistochemical results showed that the positive expression of in the nuclei of nephroblastoma tissue was 78%, among which metastasis rate was 61.7%; correspondingly, it was 8%, and 100% in adjacent tissues. The qPCR results indicated that , , and were significantly upregulated after overexpression compared with the normal embryonic tissue cells, control, and gene knockout groups ( <0.05). could cause the occurrence, development, and metastasis of childhood nephroblastoma through wingless/int1/Frizzled-related signaling pathways.
本研究旨在通过研究60例儿童肾母细胞瘤和58例副肿瘤癌病例，探讨原发性Fos相关抗原1（-1）癌基因在肾母细胞瘤中的作用。采用免疫组织化学法检测其表达。通过病毒转染进行肾母细胞瘤细胞培养，建立过表达和基因敲除模型。采用流式细胞术和纳米PCR检测相关通路基因的凋亡和mRNA表达。免疫组织化学结果显示，肾母细胞瘤组织细胞核中阳性表达率为78%，其中转移率为61.7%；相应地，在相邻组织中分别为8%和100%。qPCR结果表明，与正常胚胎组织细胞、对照组和基因敲除组相比，过表达后， 、 和 显著上调（<0.05）。 可通过无翅/int1/卷曲相关信号通路导致儿童肾母细胞瘤的发生、发展和转移。
