Department of Radiotherapy, Affiliated Hospital of Weifang Medical University, Weifang 261031, Shandong, China.
Acta Biochim Pol. 2022 Apr 28;69(2):343-348. doi: 10.18388/abp.2020_5794.
Increasing evidence suggests that the LncRNA/miRNA axis plays a key role in many types of tumorigenesis. However, the potential role and clinical value of LINC00313/miR-4429 in non-small cell lung cancer (NSCLC) remain elusive and need further study. The aim of this study was to investigate the role of LINC00313/miR-4429 axis in NSCLC.
The expression of LINC00313 and miR-4429 in serum, tissues and cell lines of NSCLC patients were detected by reverse transcription-quantitative PCR. The diagnostic value was evaluated by receiver operating characteristic (ROC) curve analysis and the prognostic value was analyzed by Kaplan-Meier survival analysis and Cox regression.
The expression of LINC00313 was significantly up-regulated while the expression of miR-4429 was down-regulated in NSCLC serum, tissue and cell lines. A negative correlation was found between LINC00313 and miR-4429 in patients with NSCLC. ROC curves showed that LINC00313 and miR-4429 had high diagnostic value and Kaplan-Meier curve results showed that high expression of LINC00313 and low expression of miR-4429 predicted poor prognosis.
In summary, our data show that the ectopic expression of LINC00313 and miR-4429 has promising clinical significance in the diagnosis and prognosis of NSCLC. The LINC00313/miR-4429 axis may provide novel biomarker for NSCLC treatment.
越来越多的证据表明，LncRNA/miRNA 轴在多种肿瘤的发生中起着关键作用。然而，LINC00313/miR-4429 在非小细胞肺癌（NSCLC）中的潜在作用和临床价值仍不清楚，需要进一步研究。本研究旨在探讨 LINC00313/miR-4429 轴在 NSCLC 中的作用。
采用逆转录定量 PCR 检测 NSCLC 患者血清、组织和细胞系中 LINC00313 和 miR-4429 的表达。通过受试者工作特征（ROC）曲线分析评估诊断价值，通过 Kaplan-Meier 生存分析和 Cox 回归分析评估预后价值。
与正常对照相比，LINC00313 在 NSCLC 血清、组织和细胞系中表达上调，miR-4429 表达下调。NSCLC 患者中 LINC00313 与 miR-4429 呈负相关。ROC 曲线表明 LINC00313 和 miR-4429 具有较高的诊断价值，Kaplan-Meier 曲线结果表明 LINC00313 高表达和 miR-4429 低表达预测预后不良。
综上所述，我们的数据表明，LINC00313 和 miR-4429 的异位表达在 NSCLC 的诊断和预后中有很好的临床意义。LINC00313/miR-4429 轴可能为 NSCLC 的治疗提供新的生物标志物。
