Faculdade Una - Unidade Jataí, Jataí, Brazil.
Universidade Federal de Goiás (UFG), Escola de Veterinária e Zootecnia (EVZ), Goiânia, Brazil.
Anat Histol Embryol. 2022 Jul;51(4):435-442. doi: 10.1111/ahe.12805. Epub 2022 Apr 29.
In this study, atomic force microscopy (AFM), microtomography (MCT-2D and MCT-3D) and energy-dispersive X-ray fluorescence spectrometry (EDXRF) were used to generate parameters of the microstructure of the hoof capsule of pigmented and partial albino buffaloes. Seventy-two digits of adult pigmented buffaloes and 16 of partial albino buffaloes were used and equally divided into thoracic and pelvic limbs and medial and lateral claws. Fragments of 10 mm × 10 mm of the dorsal wall, abaxial wall and pre-bulbar sole were collected. The parametric assumptions were tested using a Shapiro-Wilk test (normality). The independent t-test was used to compare the means at a 5% significance level. AFM demonstrated that the hoof surface of pigmented buffaloes presented with higher average surface roughness (Ra) and root mean square roughness (Rms) (p < 0.05) than the hoof surface of partial albino buffaloes. MCT-2D revealed that pigmented buffaloes had extra tubular keratin with a higher density than intratubular keratin. No pores were observed in the hoof capsule of the buffalo digits. MCT-3D demonstrated that pigmented buffaloes have a higher percentage of large and intermediate horn tubules than partial albino buffaloes. However, this difference was not statistically significant. Partial albino buffaloes showed a statistically higher number of horn tubules/mm than pigmented buffaloes (p < 0.05). EDXRF revealed a higher amount of sulphur (S) in the hoof capsule of pigmented buffaloes, and the partial albino buffaloes presented a higher number of minerals such as calcium (Ca), potassium (K), zinc (Zn) and copper (Cu).
本研究采用原子力显微镜（AFM）、二维和三维微断层扫描（MCT-2D 和 MCT-3D）以及能量色散 X 射线荧光光谱法（EDXRF），生成色素型和部分白化型水牛 hoof 壳微观结构的参数。使用了 72 个成年色素型水牛和 16 个部分白化型水牛的趾骨，平均分为胸肢和后肢，以及内侧和外侧趾爪。采集了 dorsal wall、abaxial wall 和 pre-bulbar sole 背侧壁、背侧壁和前球底 10mm×10mm 的碎片。参数假设采用 Shapiro-Wilk 检验（正态性）进行检验。独立样本 t 检验用于在 5%显著水平上比较均值。AFM 表明，色素型水牛的 hoof 表面具有更高的平均表面粗糙度（Ra）和均方根粗糙度（Rms）（p<0.05）。MCT-2D 显示，色素型水牛的 extra tubular keratin 密度高于 intratubular keratin。水牛趾骨的 hoof 壳中没有观察到孔。MCT-3D 表明，色素型水牛的 large 和 intermediate 角蛋白管比部分白化型水牛更高。然而，这种差异没有统计学意义。部分白化型水牛的 horn tubules/mm 数量高于色素型水牛（p<0.05）。EDXRF 表明色素型水牛的 hoof 壳中含有更多的硫（S），而部分白化型水牛则含有更多的钙（Ca）、钾（K）、锌（Zn）和铜（Cu）等矿物质。
