State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P.R. China.
University of Science and Technology of China, Hefei, 230026, P.R. China.
Analyst. 2022 May 17;147(10):2097-2105. doi: 10.1039/d2an00483f.
Manganese-based nanozymes have been widely used in the field of cell protection due to their various enzyme-mimicking activities, but their effect on the mechanical properties of cells is not yet known. Here, bovine serum albumin-modified MnO nanoparticles (BSA-MnO NPs) with good biocompatibility were synthesized by a one-step biomineralization method using BSA as a template. BSA-MnO NPs possess scavenging activity against superoxide free radicals (O˙), hydroxyl radicals (˙OH) and hydrogen peroxide (HO). The excellent reactive oxygen species (ROS) scavenging activity of BSA-MnO NPs enables them to effectively reduce the intracellular ROS level, thus mitigating the damage of oxidative stress on human umbilical vein endothelial cells (HUVECs). Subsequently, the intracellular antioxidant mechanism of the BSA-MnO NPs was further investigated. The results show that the BSA-MnO NPs could inhibit the depolymerization of F-actin, help cells maintain their normal morphology, and reduce the decrease in Young's modulus of cells caused by oxidative stress.
基于锰的纳米酶由于其多种酶模拟活性，已被广泛应用于细胞保护领域，但它们对细胞机械性能的影响尚不清楚。在这里，我们使用牛血清白蛋白（BSA）作为模板，通过一步生物矿化法合成了具有良好生物相容性的 MnO 纳米粒子（BSA-MnO NPs）。BSA-MnO NPs 具有清除超氧自由基（O˙）、羟基自由基（˙OH）和过氧化氢（HO）的能力。BSA-MnO NPs 具有优异的活性氧（ROS）清除活性，能够有效降低细胞内 ROS 水平，从而减轻氧化应激对人脐静脉内皮细胞（HUVECs）的损伤。随后，进一步研究了 BSA-MnO NPs 的细胞内抗氧化机制。结果表明，BSA-MnO NPs 可以抑制 F-肌动蛋白的解聚，帮助细胞维持正常形态，并减少氧化应激引起的细胞杨氏模量的降低。
