State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China; University of Science and Technology of China, Hefei, 230026, China.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China.
Anal Chim Acta. 2022 Sep 22;1227:340321. doi: 10.1016/j.aca.2022.340321. Epub 2022 Aug 28.
Nanoparticles-based antioxidative therapy has been highlighted in a series of diseases triggered by excessive reactive oxygen species (ROS). Prussian blue nanoparticles (PBNPs), as a representative artificial nanozyme, have been proved as highly effective ROS scavengers. However, its detailed intracellular antioxidant mechanism is not clear yet. Herein, a series of PBNPs with different particle sizes were synthesized and their intracellular antioxidant activities were studied by atomic force microscopy (AFM) from a biomechanical perspective. We first validated the ROS scavenging ability of PBNPs in vitro. It indicated that PBNPs had great scavenging effect on multiple ROS, such as hydroxyl radicals (•OH), superoxide radicals (O) and hydrogen peroxide (HO). By observing the changes in morphology and mechanical properties of human umbilical vascular endothelium cells (HUVECs), it was further found that PBNPs could apparently alleviate the decrease of Young's modulus caused by oxidative stress damage and kept cells in their normal morphology. In addition, the distribution of F-actin revealed that the enhancement of cytoskeleton stability by PBNPs might be a key way to protect HUVECs from oxidative damage. Importantly, the antioxidant activities of PBNPs were found to be size-dependent, which indicated the smaller particle size had better antioxidant activities compared with the larger particle size. This study serves as a novel medium to reveal the mechanism of nanoparticles on cells at the single-cell level and demonstrates the great potential of atomic force microscopy in studying the application of nanoparticles in cell biology.
基于纳米粒子的抗氧化治疗在一系列由过量活性氧物种 (ROS) 引发的疾病中得到了强调。普鲁士蓝纳米粒子 (PBNPs) 作为一种代表性的人工纳米酶，已被证明是一种非常有效的 ROS 清除剂。然而，其详细的细胞内抗氧化机制尚不清楚。在此，我们合成了一系列具有不同粒径的 PBNPs，并从生物力学的角度通过原子力显微镜 (AFM) 研究了它们的细胞内抗氧化活性。我们首先在体外验证了 PBNPs 清除 ROS 的能力。结果表明，PBNPs 对多种 ROS 具有很强的清除作用，如羟基自由基 (•OH)、超氧自由基 (O) 和过氧化氢 (HO)。通过观察人脐静脉内皮细胞 (HUVEC) 形态和力学性能的变化，进一步发现 PBNPs 可以明显减轻氧化应激损伤引起的杨氏模量降低，并使细胞保持正常形态。此外，肌动蛋白纤维的分布表明，PBNPs 增强细胞骨架稳定性可能是保护 HUVECs 免受氧化损伤的关键途径。重要的是，发现 PBNPs 的抗氧化活性与粒径有关，这表明与较大粒径相比，较小粒径的 PBNPs 具有更好的抗氧化活性。这项研究为揭示纳米粒子在单细胞水平上对细胞的作用机制提供了一种新的方法，并证明了原子力显微镜在研究纳米粒子在细胞生物学中的应用方面具有巨大的潜力。
