Kadushkin A G, Tahanovich A D, Movchan L V, Zafranskaya M M, Dziadzichkina V V, Shman T V
Belarusian State Medical University, Minsk, Belarus.
Republican Scientific and Practical Center for Pediatric Oncology, Hematology and Immunology, Minsk, Belarus.
Biomed Khim. 2022 Apr;68(2):134-143. doi: 10.18097/PBMC20226802134.
To date, there are no drugs that can prevent progressive destruction of lung tissue and small airway fibrosis in patients with chronic obstructive pulmonary disease (COPD). Therefore, molecular mechanisms of this disease are being studied. The aim of this study was to determine the chemokine receptor expression pattern of B-lymphocytes from peripheral blood and airways of patients with COPD. Peripheral blood was collected from 51 smokers with COPD, 21 healthy smokers, and 20 healthy non-smokers. Seven smokers with COPD and 7 healthy smokers were recruited to undergo bronchoscopy with bronchoalveolar lavage (BAL). The expression of chemokine receptors CCR5, CCR6, CCR7, CXCR3, CXCR4, and CXCR5 on the surface of blood and BAL B-lymphocytes was determined by flow cytometry. It was found that the percentage of blood B-lymphocytes expressing chemokine receptors CCR5 and CXCR3 was higher in smokers with COPD compared with healthy smokers and healthy non-smokers. The percentage of CD27⁺ B-cells expressing CCR5 and CXCR3 receptors exceeded the proportion of CD27⁻ B-lymphocytes expressing these receptors in peripheral blood of patients with COPD and healthy controls. In smoking patients with COPD, the percentage of BAL B-cells expressing receptors CCR5 and CXCR3 was also increased compared with healthy smokers. There were no differences in the percentage of B-lymphocytes expressing receptors CXCR4, CXCR5, CCR6, and CCR7 in both peripheral blood and BAL between smokers with COPD and healthy smokers. A greater percentage of CD27⁻ B-lymphocytes than CD27⁺ B-cells expressed receptors CXCR4, CXCR5, CCR6, and CCR7 in the peripheral blood of smokers with COPD and healthy controls. The results of this study indicate a modification in the chemokine receptor profile of B-lymphocytes in COPD.
迄今为止，尚无药物能够预防慢性阻塞性肺疾病（COPD）患者肺组织的进行性破坏和小气道纤维化。因此，正在对该疾病的分子机制进行研究。本研究的目的是确定COPD患者外周血和气道中B淋巴细胞的趋化因子受体表达模式。采集了51例患有COPD的吸烟者、21例健康吸烟者和20例健康非吸烟者的外周血。招募了7例患有COPD的吸烟者和7例健康吸烟者接受支气管镜检查及支气管肺泡灌洗（BAL）。通过流式细胞术测定血液和BAL B淋巴细胞表面趋化因子受体CCR5、CCR6、CCR7、CXCR3、CXCR4和CXCR5的表达。结果发现，与健康吸烟者和健康非吸烟者相比，患有COPD的吸烟者中表达趋化因子受体CCR5和CXCR3的血液B淋巴细胞百分比更高。在COPD患者和健康对照者的外周血中，表达CCR5和CXCR3受体的CD27⁺ B细胞百分比超过了表达这些受体的CD27⁻ B淋巴细胞比例。在患有COPD的吸烟患者中，与健康吸烟者相比，表达受体CCR5和CXCR3的BAL B细胞百分比也有所增加。在患有COPD的吸烟者和健康吸烟者的外周血和BAL中，表达受体CXCR4、CXCR5、CCR6和CCR7的B淋巴细胞百分比没有差异。在患有COPD的吸烟者和健康对照者的外周血中，表达受体CXCR4、CXCR5、CCR6和CCR7的CD27⁻ B淋巴细胞百分比高于CD27⁺ B细胞。本研究结果表明COPD患者B淋巴细胞的趋化因子受体谱发生了改变。
