Tokareva A O, Starodubtseva N L, Chagovets V V, Rodionov V V, Kometova V V, Chingin K S, Frankevich V E
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Moscow, Russia.
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Moscow, Russia; 2V.L. Talrose Institute for Energy Problems of Chemical Physics, N.N. Semenov Federal Center of Chemical Physics, Moscow, Russia.
Biomed Khim. 2022 Apr;68(2):144-152. doi: 10.18097/PBMC20226802144.
Research of cancer progression mechanisms and their impact on metabolism of tumor cells and tumor microenvironment cells is an important element in drug development for cancer target therapy. In this study, changes in tumor tissue and margin tissue lipid profiles, were associated with the following clinical and morphological characteristics: tumor size, cancer stage, multifocalite, tumor grade, number of lymph node metastasis, Nottingham prognostic index, total malignancy score, level of Ki67 protein. Lipid profiling was performed by reverse-phase chromato-mass spectrometry analysis of lipid tissue extract with lipid identification by characteristic fragments. In the lipid profile of tumor tissue 13 characteristic lipids were selected. Their levels significantly correlated with at least 5 clinical and morphological features. Eight of 13 belonged to phosphatidylcholines. In lipid profile of tumor microenviroment tissue 13 lipid features were selected. Their levels significantly correlated with at least 5 clinical and morphological features. Four of 13 belonged to oxidized lipids, 4 lipid features belonged to sphingomyelins, four of 13 belonged to phosphatidylethanolamines. The tumor microenvironment tissue lipid profile correlated with tumor size, cancer stage, tumor grade, number of axillary metastases, Nottingham prognostic index. The tumor tissue lipid profile correlated with tumor size, tumor grade, total malignant score, and number of axillary metastases.
癌症进展机制及其对肿瘤细胞和肿瘤微环境细胞代谢的影响的研究是癌症靶向治疗药物开发的重要组成部分。在本研究中，肿瘤组织和边缘组织脂质谱的变化与以下临床和形态学特征相关：肿瘤大小、癌症分期、多灶性、肿瘤分级、淋巴结转移数量、诺丁汉预后指数、总恶性评分、Ki67蛋白水平。脂质谱分析通过对脂质组织提取物进行反相色谱-质谱分析，并通过特征性碎片进行脂质鉴定。在肿瘤组织的脂质谱中，选择了13种特征脂质。它们的水平与至少5种临床和形态学特征显著相关。13种中有8种属于磷脂酰胆碱。在肿瘤微环境组织的脂质谱中，选择了13种脂质特征。它们的水平与至少5种临床和形态学特征显著相关。13种中有4种属于氧化脂质，4种脂质特征属于鞘磷脂，13种中有4种属于磷脂酰乙醇胺。肿瘤微环境组织脂质谱与肿瘤大小、癌症分期、肿瘤分级、腋窝转移数量、诺丁汉预后指数相关。肿瘤组织脂质谱与肿瘤大小、肿瘤分级、总恶性评分和腋窝转移数量相关。
