Internal Medicine Department, Beni-Suef University, Egypt.
Clinical Pathology Department, Beni-Suef University, Egypt.
Asian Pac J Cancer Prev. 2022 Apr 1;23(4):1373-1377. doi: 10.31557/APJCP.2022.23.4.1373.
Hepatocellular carcinoma (HCC) is a common cancer with substantial cancer-related deaths worldwide. Deregulation of some genetic polymorphisms has been identified in HCC.
We aimed to demonstrate the frequency of miRNA 196a2 rs11614913 and miRNA 34 b/c rs4938723 gene polymorphisms in HCC patients and their correlation with the clinical features and laboratory findings at diagnosis.
The study was performed on 40 patients with newly diagnosed HCC and 40 patients with liver cirrhosis in addition to 40 age and sex-matched healthy controls. Detection of miRNA 196a2 rs11614913 and miRNA 34 b/c rs4938723 gene polymorphisms was determined by PCR-RFLP.
HCC patients had significantly higher frequency of miR-196-2a rs11614913 CC genotype when compared with cirrhotic patients (60.0 % versus 30.0 %, p=0.013). In spite of the fact that HCC patients also had higher frequency of miR-196-2a rs11614913 CC genotype in comparison to controls, the difference fell short of statistical significance (60.0 % versus 42.5 %, p=0.18). No significant differences were found between the studied groups regarding the frequency of miR-196-2a alleles. miR34 b/c rs4938723 CC genotype was the only identified genotype in all participants in the three studied groups. No significant associations were found between the different clinical and laboratory variables and genotypic variations in HCC patients.
This study identified miR-196a2 rs11614913 CC genotype as a risk factor for HCC development while we failed to document similar relation for miR-34b/c rs4938723 polymorphism.
肝细胞癌（HCC）是一种常见的癌症，在全球范围内导致了大量与癌症相关的死亡。已经发现一些遗传多态性的失调与 HCC 有关。
我们旨在展示 HCC 患者中 miRNA 196a2 rs11614913 和 miRNA 34b/c rs4938723 基因多态性的频率及其与诊断时临床特征和实验室发现的相关性。
该研究纳入了 40 例新诊断的 HCC 患者、40 例肝硬化患者和 40 例年龄和性别匹配的健康对照者。通过 PCR-RFLP 检测 miRNA 196a2 rs11614913 和 miRNA 34b/c rs4938723 基因多态性。
与肝硬化患者相比，HCC 患者 miR-196-2a rs11614913 CC 基因型的频率显著更高（60.0% 对 30.0%，p=0.013）。尽管与对照组相比，HCC 患者 miR-196-2a rs11614913 CC 基因型的频率也更高，但差异无统计学意义（60.0% 对 42.5%，p=0.18）。在三个研究组中，miR-196-2a 等位基因的频率在研究组之间没有显著差异。miR34b/c rs4938723 CC 基因型是所有参与者在三个研究组中唯一发现的基因型。在 HCC 患者中，不同的临床和实验室变量与基因型变异之间没有发现显著关联。
本研究确定 miR-196a2 rs11614913 CC 基因型是 HCC 发生的危险因素，而我们未能证明 miR-34b/c rs4938723 多态性存在类似关系。
