College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China; Ningbo Research Institute, Zhejiang University, Ningbo 315100, China.
Int J Biol Macromol. 2022 Jun 15;210:465-474. doi: 10.1016/j.ijbiomac.2022.04.155. Epub 2022 Apr 26.
In this study, zanthoxylum bungeanum essential oil/β-cyclodextrin inclusion complexes (ZBEO/β-CD-ICs) were first prepared by precipitation method. When the addition of ZBEO was 1 g, the reaction time was 4 h and the reaction temperature was 55 °C, the recovery (73.88%) and loading content (9.53%) reached the highest value. The characterization results showed inclusion complexation changed the crystalline structure, enhanced interaction among molecules and increased the thermal stability. Then, nanofiber films containing ZBEO/β-CD-ICs were prepared by electrospinning. When the total polymer concentration was constant at 20%, with the increase of ZBEO/β-CD-IC content, the diameter of nanofiber and mechanical strength decreased, but the temperature corresponding to the maximum rate of weight loss increased. X-ray diffraction analysis proved that the addition of ZBEO/β-CD-IC increased the crystallinity degree of film. The Fourier transform infrared spectra indicated hydrogen bond interactions among molecules. Releasing behavior of ZBEO indicated that increase of temperature and relative humidity accelerated the releasing speed. Antibacterial and antioxidant activity results demonstrated the increase of ZBEO content enhanced antibacterial and antioxidant efficiency, Z40P10 nanofibers had the maximum antibacterial rate of 62.02% against S. aureus and the maximum antioxidant activity of 60.18%.
在这项研究中，首次通过沉淀法制备了花椒精油/β-环糊精包合物（ZBEO/β-CD-ICs）。当添加 ZBEO 为 1g、反应时间为 4h、反应温度为 55°C 时，回收率（73.88%）和载药量（9.53%）达到最高值。表征结果表明，包合作用改变了晶体结构，增强了分子间的相互作用，提高了热稳定性。然后，通过静电纺丝制备了含有 ZBEO/β-CD-IC 的纳米纤维膜。当总聚合物浓度恒定时为 20%，随着 ZBEO/β-CD-IC 含量的增加，纳米纤维的直径和机械强度降低，但最大失重速率对应的温度升高。X 射线衍射分析证明了 ZBEO/β-CD-IC 的添加增加了膜的结晶度。傅里叶变换红外光谱表明分子间存在氢键相互作用。ZBEO 的释放行为表明，温度和相对湿度的升高加速了释放速度。抗菌和抗氧化活性结果表明，ZBEO 含量的增加提高了抗菌和抗氧化效率，Z40P10 纳米纤维对金黄色葡萄球菌的最大抑菌率为 62.02%，最大抗氧化活性为 60.18%。
