Vazquez Dana V, Pereira da Costa Javier H, Godoy Federico N I, Cambiaso Vladimir, Rodríguez Gustavo R
Instituto de Investigaciones en Ciencias Agrarias de Rosario (IICAR-CONICET-UNR), Campo Experimental Villarino, S2125ZAA Zavalla, Santa Fe, Argentina; Cátedra de Genética, Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, Campo Experimental Villarino, S2125ZAA Zavalla, Santa Fe, Argentina.
Instituto de Investigaciones en Ciencias Agrarias de Rosario (IICAR-CONICET-UNR), Campo Experimental Villarino, S2125ZAA Zavalla, Santa Fe, Argentina.
Plant Sci. 2022 Jun;319:111258. doi: 10.1016/j.plantsci.2022.111258. Epub 2022 Mar 16.
Fruit shape is a key trait in tomato (Solanum lycopersicum L.). Since most studies focused on proximo-distal fruit morphology, we hypothesized that unknown QTLs for medio-lateral direction ones could be found analysing segregating populations where major shape genes are fixed. We examined the diversity of fruit morphology in medio-lateral direction; defined divergent traits in cultivars carrying identical genetic constitution at LC and FAS genes; and identified QTLs for lobedness degree (LD) by a QTL-seq approach. We found that LC and FAS genes were not enough to explain LD variability in a large tomato collection. Then, we derived F populations crossing cultivars divergent for LD where LC and FAS were fixed (Yellow Stuffer x Heinz 1439 [FYSxH] and Voyage x Old Brooks [FVxOB]). By QTL-seq we identified a QTL for LD on chromosome 8 in both F, which was validated in FYSxH by interval mapping accounting for ~ 17% of the variability. Other two QTLs located on chromosomes 6 and 11 with epistasis explained ~ 61% of the variability in the FVxOB. In conclusion, three novel QTLs with major effect for LD (ld6, ld8, and ld11) were identified through the study of diversity and genetic segregation in intraspecific tomato crosses.
果实形状是番茄（Solanum lycopersicum L.）的一个关键性状。由于大多数研究集中在果实近远轴形态上，我们推测通过分析主要形状基因固定的分离群体，可能会发现影响果实中外侧方向的未知数量性状基因座（QTL）。我们研究了果实中外侧方向的形态多样性；在LC和FAS基因具有相同遗传构成的品种中定义了不同的性状；并通过QTL-seq方法鉴定了叶裂程度（LD）的QTL。我们发现，在一个大型番茄群体中，LC和FAS基因不足以解释LD的变异性。然后，我们通过杂交在LC和FAS固定的情况下LD不同的品种获得了F群体（Yellow Stuffer × Heinz 1439 [FYSxH]和Voyage × Old Brooks [FVxOB]）。通过QTL-seq，我们在两个F群体中均在8号染色体上鉴定出一个LD的QTL，并通过区间作图在FYSxH中进行了验证，该QTL解释了约17%的变异性。位于6号和11号染色体上的另外两个具有上位性的QTL解释了FVxOB中约61%的变异性。总之，通过对种内番茄杂交的多样性和遗传分离研究，鉴定出了三个对LD有主要影响的新QTL（ld6、ld8和ld11）。
