Brewer Marin Talbot, Moyseenko Jennifer B, Monforte Antonio J, van der Knaap Esther
Department of Horticulture and Crop Science, The Ohio State University/OARDC, 1680 Madison Avenue, Wooster, OH 44691, USA.
J Exp Bot. 2007;58(6):1339-49. doi: 10.1093/jxb/erl301. Epub 2007 Feb 5.
Variation in fruit morphology is a prevalent characteristic among cultivated tomato. The genetic and developmental mechanisms underlying similarities and differences in shape between the fruit of two elongated tomato varieties were investigated. Fruit from two F2 populations constructed from either Solanum lycopersicum cv. Howard German or cv. Banana Legs crossed with S. pimpinellifolium accession LA1589, and one BC1 population constructed with S. lycopersicum Howard German as the recurrent parent, were analysed for shape by using a new software program Tomato Analyzer. Quantitative trait loci (QTLs) controlling 15 individual shape attributes were mapped by both single and multitrait composite interval mapping in each population. In addition, principal components analysis and canonical discriminant analysis were conducted on these shape attributes to determine the greatest sources of variation among and between the populations. Individual principal components and canonical variates were subjected to QTL analysis to map regions of the genome influencing fruit shape in the cultivars. Common and unique regions, as well as previously known and novel QTLs, underlying fruit morphology in tomato were identified. Four major loci were found to control multiple fruit shape traits, principal components, and canonical variates in the populations. In addition, QTLs associated with the principal components better revealed regions of the genome that varied among populations than did the QTL associated with canonical variates. The QTL identified can be compared across additional populations of tomato and other fruit-bearing crop species.
果实形态变异是栽培番茄的一个普遍特征。研究了两个长形番茄品种果实形状异同的遗传和发育机制。利用一个新的软件程序“番茄分析仪”，对由栽培番茄品种“霍华德德国种”或“香蕉腿”与醋栗番茄种质LA1589构建的两个F2群体，以及以“霍华德德国种”为轮回亲本构建的一个BC1群体的果实形状进行了分析。通过单性状和多性状复合区间作图，在每个群体中定位了控制15个个体形状属性的数量性状位点（QTL）。此外，对这些形状属性进行了主成分分析和典型判别分析，以确定群体间和群体内最大的变异来源。对个体主成分和典型变量进行QTL分析，以定位影响品种果实形状的基因组区域。确定了番茄果实形态的共同和独特区域，以及先前已知和新发现的QTL。发现四个主要位点控制群体中的多个果实形状性状、主成分和典型变量。此外，与主成分相关的QTL比与典型变量相关的QTL能更好地揭示群体间基因组的变异区域。所鉴定的QTL可在其他番茄群体和其他结果作物物种中进行比较。
