Anđelković Milena, Djordjevic Aleksandra Buha, Javorac Dragana, Baralić Katarina, Đukić-Ćosić Danijela, Repić Aleksandra, Zeljković Aleksandra, Vekić Jelena, Čolaković Nataša, Bulat Zorica
University Hospital Medical Center Kosovska Mitrovica, Kosovska Mitrovica, 38220, Serbia.
Department of Toxicology "Akademik Danilo Soldatović", University of Belgrade - Faculty of Pharmacy, Vojvode Stepe 450, Belgrade, 11221, Serbia.
Environ Sci Pollut Res Int. 2022 Sep;29(43):65211-65221. doi: 10.1007/s11356-022-20439-z. Epub 2022 Apr 29.
Numerous risk factors have been associated with breast cancer (BC), exposure to metalloestrogen, like lead, being such. Since lead involvement in BC is still equivocal, we focused on lead levels in three compartments of BC patients, blood, healthy, and malignant tissues. Also, as the cholesterol role in cancer development was recognized at the beginning of the twentieth century and led to involvement in lipid profile impairment, we further extend our research on lipid profile and enzymes responsible for maintaining lipid balance in BC patients. Fifty-five women diagnosed with BC were enrolled in the study. Forty-one healthy women represented the control group. Lead levels in blood, healthy surrounding and malignant tissue, and lipid profile parameters in serum, were determined. Higher lead levels were obtained in surrounding healthy tissue samples compared to cancerous tissue samples, while blood lead levels of BC women did not differ significantly from the control group. The altered lipid profile scheme in women diagnosed with breast cancer contained significantly higher triglycerides levels (P < 0.001). Moreover, logistic regression analysis revealed triglycerides as a significant predictor of BC (OR = 2.6; P < 0.01). Although statistical significance was missing for lower paraoxonase-1 (PON-1) activities observed in BC women, multivariate logistic regression singled out PON-1 activities as significant BC predictors. The result of the present study further indicated oxidative status imbalance and tissue levels bioelements perturbation. Obtained results in the present study propose possible lead involvement in BC onset accompanied with bioelements redistribution and oxidative stress occurrence.
众多风险因素与乳腺癌（BC）相关，接触金属雌激素（如铅）便是其中之一。由于铅与乳腺癌的关系仍不明确，我们聚焦于乳腺癌患者血液、健康组织和恶性组织这三个部位的铅含量。此外，鉴于胆固醇在癌症发展中的作用在20世纪初就已被认识到，且其与脂质代谢异常有关，我们进一步拓展了对乳腺癌患者脂质谱以及负责维持脂质平衡的酶的研究。55名被诊断为乳腺癌的女性参与了该研究。41名健康女性作为对照组。测定了血液、健康周围组织和恶性组织中的铅含量，以及血清中的脂质谱参数。与癌组织样本相比，健康周围组织样本中的铅含量更高，而乳腺癌女性的血铅水平与对照组无显著差异。被诊断为乳腺癌的女性脂质谱改变模式显示甘油三酯水平显著升高（P < 0.001）。此外，逻辑回归分析显示甘油三酯是乳腺癌的显著预测指标（OR = 2.6；P < 0.01）。尽管乳腺癌女性中观察到的对氧磷酶-1（PON-1）活性降低缺乏统计学意义，但多变量逻辑回归将PON-1活性选为乳腺癌的显著预测指标。本研究结果进一步表明氧化状态失衡和组织生物元素扰动。本研究获得的结果表明铅可能参与乳腺癌的发病，并伴有生物元素重新分布和氧化应激的发生。
