Sonia Romisha, Shaheen Shabnum, Khalid Sana, Sharifi-Rad Javad, Shahid Muhammad Naveed, Mukhtar Hanan, Khalid Zaryab, Harun Nidaa, Hussain Rana Abrar, Khan Farah
Department of Botany, Lahore College for Women University, Lahore, Pakistan.
Phytochemistry Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Microsc Res Tech. 2022 Aug;85(8):2848-2856. doi: 10.1002/jemt.24133. Epub 2022 Apr 29.
Eclipta alba (L.) is a valuable medicinal plant. However, its medicinal efficacy can be affected by geminivirus infection. Therefore, identification of healthy specimen is essential before to use as medicine. The present study provided the taxonomic characterization of geminivirus infected and healthy E. alba plant by studying apparent morphology and microscopic features through light and scanning electron microscopy. Before taxonomic characterization infected and healthy specimens were separated through molecular detection of geminivirus. Results of morphological studies reported that geminivirus infected E. alba plant showed systematic symptoms of infection like stunted growth, distortion and chlorosis of leaves, decrease in size of root, shoot and fruit, and so forth in comparison to healthy specimen. Anatomical findings reported that in both plants anomocytic and anisocytic types of stomata with multicellular warty trichomes were present. However, variations were observed in quantitative measures such as size of trichomes, epidermal, subsidiary and guard cells. Palynological observations identifies that both plants possessed tricolporate type of pollen but variation was mainly observed in size and shape of pollen, thickness of exine and intine, P/E ratio, pore size, interspecific difference, size of colpi, and pollen ornamentation. Overall this study concluded that both healthy and infected E. alba do not reported much variations in qualitative taxonomic features, but can be differentiated in terms of quantitative taxonomic evidences. Future studies are recommended for pharmacological analysis of both healthy and virus infected plants. RESEARCH HIGHLIGHTS: Eclipta alba has incredible therapeutic worth, but due to geminivirus infection the plant is affecting badly. Hence, the present studies give a comprehensive taxonomic report on the geminivirus infected and healthy plant species.
白花蟛蜞菊是一种珍贵的药用植物。然而，其药用功效会受到双生病毒感染的影响。因此，在用作药物之前识别健康的标本至关重要。本研究通过光学显微镜和扫描电子显微镜研究明显形态和微观特征，对感染双生病毒和健康的白花蟛蜞菊植株进行了分类学特征描述。在进行分类学特征描述之前，通过双生病毒的分子检测将感染和健康的标本分开。形态学研究结果表明，与健康标本相比，感染双生病毒的白花蟛蜞菊植株表现出系统性感染症状，如生长受阻、叶片扭曲和黄化、根、茎和果实尺寸减小等。解剖学研究结果表明，两种植株均存在不规则型和不等细胞型气孔以及多细胞疣状毛状体。然而，在毛状体、表皮、副卫细胞和保卫细胞的大小等定量测量方面观察到了差异。孢粉学观察表明，两种植株均具有三孔沟型花粉，但主要在花粉的大小和形状、外壁和内壁厚度、P/E 比、孔径、种间差异、沟的大小和花粉纹饰方面观察到了变化。总体而言，本研究得出结论，健康和感染的白花蟛蜞菊在定性分类学特征方面没有太多差异，但可以根据定量分类学证据进行区分。建议未来对健康和病毒感染植株进行药理学分析研究。研究亮点：白花蟛蜞菊具有令人难以置信的治疗价值，但由于双生病毒感染，该植物受到严重影响。因此，本研究给出了关于感染双生病毒和健康植物物种的全面分类学报告。
