Nogueira M E, Mendes R P, Marques S A, Franco M
Braz J Med Biol Res. 1986;19(2):241-7.
The objective of this study was to detect the presence of antibodies by the complement-mediated lysis test in patients with paracoccidioidomycosis, before and after treatment, and to correlate them with the clinical form of the disease and with the levels of precipitin and fluorescent anti-P. brasiliensis antibodies. Eighty percent of sera from 15 untreated paracoccidioidomycosis patients showed positive lytic activity indices (greater than or equal to 15%), as opposed to 50% of sera from 24 treated patients. Sera from 29 of 30 control group blood donors showed 0 to 14% lysis. No correlation was observed between lytic antibody levels and precipitin titers or anti-P. brasiliensis total Ig and IgM antibody titers, either in the untreated or treated patient group. Anti-P. brasiliensis lytic antibodies were detected in various clinical forms of paracoccidioidomycosis. This is the first study using living forms of the fungus to detect anti-P. brasiliensis antibodies and opens the possibility of using the lytic antibodies as indicators of active disease.
本研究的目的是通过补体介导的溶血试验检测副球孢子菌病患者治疗前后抗体的存在情况，并将其与疾病的临床类型以及沉淀素和荧光抗巴西副球孢子菌抗体水平相关联。15例未经治疗的副球孢子菌病患者中，80%的血清显示出阳性溶血活性指数（大于或等于15%），而24例接受治疗患者的血清中这一比例为50%。30名对照组献血者中，29人的血清溶血率为0%至14%。在未经治疗或治疗后的患者组中，均未观察到溶血抗体水平与沉淀素滴度或抗巴西副球孢子菌总Ig和IgM抗体滴度之间存在相关性。在副球孢子菌病的各种临床类型中均检测到了抗巴西副球孢子菌溶血抗体。这是首次使用真菌的活形式检测抗巴西副球孢子菌抗体的研究，并开启了将溶血抗体用作活动性疾病指标的可能性。
