Federal Center for Animal Health, Vladimir, Russia.
Agricultural Research Council, Onderstepoort Veterinary Institute, Pretoria, South Africa.
Transbound Emerg Dis. 2022 Sep;69(5):e2312-e2317. doi: 10.1111/tbed.14574. Epub 2022 May 20.
Lumpy skin disease virus causes a debilitating pox disease of domesticated cattle and water buffalos. In the last decade, LSDV has spread from Africa into the Middle East, Europe and most recently Asia. As of 2017, atypical outbreaks caused by novel LSDV strains were reported in Russia, followed by China and Vietnam between 2018 and 2020. In this work, we describe another unique recombinant LSDV strain recovered from Tyumen, Russia in 2019. Typing of the virus using currently available qPCR protocols produced inconclusive results and subsequently the complete genome of the isolate was determined. The consensus genome contained statistically significant signals of possible recombination events between parental strains KSGPO-240/Kenya/1958 and the live attenuated vaccine LW/1958. The novel strain carries 25 unique breakpoints different from the known recombinant strains. Additionally, the findings reiterate the importance of complete genome sequencing when analysing outbreak samples caused in particular by mosaic LSDV, in contrast to only performing specified qPCRs.
块状皮肤病病毒导致家养牛和水牛患上严重的痘病。在过去十年中，块状皮肤病病毒已从非洲传播到中东、欧洲，最近又传播到亚洲。截至 2017 年，俄罗斯报告了由新型块状皮肤病病毒引起的非典型暴发，随后 2018 年至 2020 年间中国和越南也报告了这种情况。在这项工作中，我们描述了 2019 年从俄罗斯秋明地区分离出的另一种独特的重组块状皮肤病病毒株。使用目前可用的 qPCR 方案对病毒进行分型产生了不确定的结果，随后确定了该分离株的完整基因组。该共识基因组包含了父母株 KSGPO-240/肯尼亚/1958 和活减毒疫苗 LW/1958 之间可能发生重组事件的统计上显著信号。与已知的重组株相比，新型株携带 25 个独特的断点。此外，这些发现再次强调了在分析特别是由镶嵌型块状皮肤病病毒引起的暴发样本时进行完整基因组测序的重要性，而不仅仅是进行特定的 qPCR。
