Gall C, Lauterborn J, Burks D, Seroogy K
Brain Res. 1987 Feb 17;403(2):403-8. doi: 10.1016/0006-8993(87)90085-0.
A double-label immunofluorescence technique was used to demonstrate that immunoreactivities for the functionally antagonistic neuropeptides enkephalin and cholecystokinin octapeptide (CCK) are co-localized within individual neurons and processes in discrete areas of rat midbrain and forebrain. Coexistence was most prominent within varicose pericellular axons extending from the periaqueductal gray matter to a field overlying the medial lemniscus, axons and terminal-like puncta in the central medial, paracentral, interanterodorsal and ventral anterior thalamic nuclei, and perikarya and proximal axonal fragments in layers II and III of neo- and allocortex, and in the anterior olfactory nucleus. The former two systems of axons lie in areas of spinothalamic tract termination. These data suggest that some of the antagonism of opioid analgesia by CCK occurs at the synaptic level in nociceptive areas of brain-stem and thalamus where CCK and enkephalin are co-localized and presumably co-released.
采用双标免疫荧光技术来证明，在大鼠中脑和前脑的离散区域中，功能拮抗的神经肽脑啡肽和八肽胆囊收缩素（CCK）的免疫反应性共定位于单个神经元和神经突起内。共存在从导水管周围灰质延伸至内侧丘系上方区域的曲张性细胞周轴突内最为显著，在丘脑中央内侧核、中央旁核、前背内侧核和腹前核中的轴突和终末样小点内，以及在新皮质和旧皮质的II层和III层以及前嗅核中的神经元胞体和近端轴突片段内。前两个轴突系统位于脊髓丘脑束终末区域。这些数据表明，CCK对阿片类镇痛的部分拮抗作用发生在脑干和丘脑的伤害性感受区域的突触水平，在这些区域CCK和脑啡肽共定位且可能共同释放。
