Innis R B, Corrêa F M, Uhl G R, Schneider B, Snyder S H
Proc Natl Acad Sci U S A. 1979 Jan;76(1):521-5. doi: 10.1073/pnas.76.1.521.
Cholecystokinin octapeptide-like (CCK-OP-like) immunoreactivity was localized in the rat brain by using the indirect immunofluorescence method. Specificity in immunohistochemical studies was demonstrated by the virtual elimination of staining with either preimmune sera or sera preadsorbed with CCK-OP and by the achievement of similar fluorescent patterns with two different primary anti-CCK-OP sera. CCK-OP-like fluorescence was localized in neuronal cell bodies, fibers, and varicose terminals. The most dense collections of CCK-OP cells occurred in the periaqueductal gray and in the dorsomedial hypothalamus. Substantial numbers of cells and fibers also were present in the medial/dorsal and perirhinal cortex; more limited groups of cells were found in the pyramidal layer of the hippocampus and in the dorsal raphe.
采用间接免疫荧光法对大鼠脑内的胆囊收缩素八肽样（CCK-OP样）免疫反应性进行定位。免疫组织化学研究中的特异性通过以下方式得以证明：用免疫前血清或用CCK-OP预吸附的血清进行染色时几乎没有染色，并且用两种不同的抗CCK-OP一抗血清获得了相似的荧光模式。CCK-OP样荧光定位于神经元细胞体、纤维和曲张终末。CCK-OP细胞最密集的聚集出现在导水管周围灰质和下丘脑背内侧。内侧/背侧和梨状皮质中也存在大量的细胞和纤维；在海马锥体细胞层和中缝背核中发现的细胞群较少。
