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河七鳃鳗(Lampetra fluviatilis)躯干肌肉组织中的氧化酶和肌球蛋白-ATP酶

Oxidative enzymes and myosin-ATPase in the trunk musculature of the river lamprey (Lampetra fluviatilis).

作者信息

Meyer W

出版信息

Histochem J. 1979 Mar;11(2):187-95. doi: 10.1007/BF01002995.

Abstract

The distribution and activities of several oxidative enzymes and of myosin-ATPase have been studied in the trunk musculature of the river lamprey. The strongest activity of oxidative enzymes and of myosin-ATPase was shown by the parietal fibre bundles of the myotomal subunits, whilst the central fibres reacted to a lesser extent. Pre-incubation of sections in media containing formaldehyde and glycine or with buffers of different pH, followed by incubation for myosin-ATPase, also differentiated the muscle fibres into a specific distribution pattern within the myotomes, resembling that of the oxidative enzymes to some extent. The parietal fibres were found to be of the slow type (type I) and the cental fibres to be fast (type II A).

摘要

对河七鳃鳗躯干肌肉组织中几种氧化酶和肌球蛋白 - ATP酶的分布及活性进行了研究。氧化酶和肌球蛋白 - ATP酶的最强活性出现在肌节亚单位的体壁纤维束中,而中央纤维的反应程度较小。将切片在含有甲醛和甘氨酸的培养基中或用不同pH值的缓冲液预孵育,随后进行肌球蛋白 - ATP酶孵育,也能使肌纤维在肌节内形成特定的分布模式,在一定程度上类似于氧化酶的分布模式。发现体壁纤维为慢肌型(I型),中央纤维为快肌型(II A型)。

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