Salthouse T N, Matlaga B F
Surg Gynecol Obstet. 1976 Apr;142(4):544-50.
Enzyme histochemical procedures for both hydrolase and oxidoreductase enzyme activity were applied to cryostat sections of polyglactin 910 suture implant sites. Sutures were implanted either solely in tissue or in a combination of in vitro incubation followed by implantation in vivo for total time periods of seven to 56 days. Suture absorption rates were also measured. It is concluded from the results that neither cellular nor enzyme activity is necessary for the degradation and absorption of polyglactin 910 sutures. This conclusion is based on similar absorption rates for sutures implanted solely in vivo and sutures treated in vitro and then implanted in tissue to give equivalent time spans. There were strong indications, however, that the products of suture hydrolysis are probably metabolized through the oxidative enzyme systems of cells adjacent to the suture. This mechanism of polyglactin 910 suture absorption is quite different from that observed and reported for catgut absorbable sutures.
将水解酶和氧化还原酶活性的酶组织化学方法应用于聚乙醇酸910缝线植入部位的低温切片。缝线要么单独植入组织中，要么先进行体外孵育，然后再植入体内，总时长为7至56天。同时还测量了缝线的吸收速率。结果表明，聚乙醇酸910缝线的降解和吸收既不需要细胞也不需要酶活性。这一结论基于单独在体内植入的缝线与体外处理后再植入组织以获得相同时间跨度的缝线具有相似的吸收速率。然而，有强烈迹象表明，缝线水解产物可能通过缝线附近细胞的氧化酶系统进行代谢。聚乙醇酸910缝线的这种吸收机制与可吸收肠线所观察到和报道的机制截然不同。
