State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha, PR China.
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Sep 5;277:121259. doi: 10.1016/j.saa.2022.121259. Epub 2022 Apr 27.
Integrating the advantages of catalytic hairpin components and multi-foot DNA walker, we designed a 16S rRNA detection probe template for pathogen bacteria, which utilizes DNA ligation quencher and dual catalytic hairpin@DNA walker to induce signal recovery. The dual catalytic hairpin@DNA walker uses the walking position of the target on the AuNP as a foothold to promote the reaction, so that the biosensing of the low-abundance target sequence can induce signal recovery. During the entire experiment, no enzyme is required, which can avoid the limitation of enzyme degradation under unfavorable conditions and the inability to detect the target. Most importantly, the detection template has the advantages of high sensitivity, and its detection limit is significantly better than that of single hairpin DNA walker probe. As the detection system can sensitively and rapidly detect its targeted bacteria and not rely on any enzyme and sophisticated instrumentation, it has great potential for sensitive and specific pathogenic bacteria detection.
融合催化发夹组件和多足 DNA walker 的优势，我们设计了一种用于病原体细菌的 16S rRNA 检测探针模板，该模板利用 DNA 连接淬灭剂和双催化发夹@DNA walker 诱导信号恢复。双催化发夹@DNA walker 利用 AuNP 上目标的行走位置作为立足点来促进反应，从而使低丰度目标序列的生物传感能够诱导信号恢复。在整个实验过程中，不需要酶，这可以避免在不利条件下酶降解的限制和无法检测目标。最重要的是，检测模板具有高灵敏度的优势，其检测限明显优于单发夹 DNA walker 探针。由于检测系统可以灵敏快速地检测其靶向细菌，并且不依赖任何酶和复杂的仪器，因此它在敏感和特异性致病菌检测方面具有很大的潜力。
