Collaborative Innovation Center of Technology and Equipment for Biological Diagnosis and Therapy in Universities of Shandong, Institute for Advanced Interdisciplinary Research (iAIR), University of Jinan, Jinan 250022, P.R. China.
State Key Laboratory of Crystal Materials, Shandong University, Jinan 250100, PR China.
Dalton Trans. 2023 Nov 28;52(46):17340-17348. doi: 10.1039/d3dt02450d.
As an important biomarker, microRNAs (miRNAs) play an important role in gene expression, and their detection has attracted increasing attention. In this study, a DNAzyme walker that could provide power to perform autonomous movement was designed. Based on the continuous mechanical motion characteristics of DNAzyme walker, a miRNA detection strategy for the self-assembly of AuNPs induced by the hairpin probe-guided DNAzyme walker "enzyme cleavage and walk" was established. In this strategy, DNAzyme walker continuously cleaved and walked on the hairpin probe on the surface of AuNPs to induce the continuous shedding of some segments of the hairpin probe. The remaining hairpin sequences on the surface of the AuNP pair with each other, causing the nanoparticles to self-assemble. This strategy uses the autonomous movement mechanism of DNAzyme walker to improve reaction efficiency and avoid the problem of using expensive and easily degradable proteases. Secondly, using dynamic light scattering technology as the signal output system, ultra-sensitive detection with a detection limit of 3.6 fM is achieved. In addition, this strategy has been successfully used to analyze target miRNAs in cancer cell samples.
作为一种重要的生物标志物，microRNAs（miRNAs）在基因表达中发挥着重要作用，其检测受到了越来越多的关注。本研究设计了一种 DNA zyme walker，它可以提供动力来进行自主运动。基于 DNAzyme walker 的连续机械运动特性，建立了一种 miRNA 检测策略，用于发夹探针引导的 DNAzyme walker“酶切和行走”诱导的 AuNPs 自组装。在该策略中，DNAzyme walker 不断地在 AuNPs 表面的发夹探针上进行酶切和行走，诱导发夹探针的一些片段连续脱落。AuNP 表面剩余的发夹序列彼此配对，导致纳米颗粒自组装。该策略利用 DNAzyme walker 的自主运动机制来提高反应效率，避免使用昂贵且易降解的蛋白酶的问题。其次，使用动态光散射技术作为信号输出系统，实现了检测限低至 3.6 fM 的超灵敏检测。此外，该策略已成功用于分析癌细胞样品中的靶 miRNAs。
