Vanadium nitride@carbon nanofiber composite: Synthesis, cascade enzyme mimics and its sensitive and selective colorimetric sensing of superoxide anion.

Nanozymes featuring with favorable activity, good stability and easy scale-up production, are promising to replace natural enzymes for various applications. However, it remains a challenge to explore the cascade reactions of multi-enzyme mimics, aiming at synergistic catalysis for various applications. Herein, vanadium nitride nanoparticles deposited on carbon nanofibers (VN@CNFs) composite was facilely prepared by typical electrospinning route with subsequently ammonia reduction process. The nanocomposite showed excellent peroxidase (POD)-like and superoxide dismutase (SOD)-like activities. Additionally, their catalytic mechanisms were systematically researched. Coupling of SOD-like with POD-like as cascade enzyme, a selective and sensitive colorimetric detection of superoxide anion (O) was explored, which has two linear parts, 0.05-30 μM and 30-250 μM O with the LOD of 0.0167 μM (S/N = 3). The as-proposed method was applicable to practical samples detection with satisfactory accuracy and recovery. Therefore, the VN@CNFs composite shows great prospect in biosensing, superoxide anion removal and biocatalysis.