Gunma University Heavy Ion Medical Center, Gunma, Japan;
Gunma University Heavy Ion Medical Center, Gunma, Japan.
Anticancer Res. 2022 May;42(5):2469-2477. doi: 10.21873/anticanres.15725.
BACKGROUND/AIM: This study aimed to establish a setup for ultra-high-dose-rate (FLASH) carbon-ion irradiation, and to conduct the first human cell experiments using FLASH carbon ions.
A system for FLASH carbon-ion irradiation (1-3 Gy at 13 or 50 keV/μm) was developed. The growth and senescence of HFL1 lung fibroblasts were assessed by crystal violet staining assays and senescence-associated β-galactosidase staining, respectively. Survival of HSGc-C5 cancer cells was assessed by clonogenic assays.
The dose rates of carbon ions ranged from 96-195 Gy/s, meeting the definition of FLASH. With both 13 and 50 keV/μm beams, no FLASH sparing effect was observed on the growth suppression and senescence of HFL1 cells, nor on the survival of HSGc-C5 cells.
We successfully conducted the first human cell experiments with FLASH carbon ions. No FLASH effect was observed under the conditions examined.
背景/目的：本研究旨在建立超高速率（FLASH）碳离子照射的设置，并进行首次使用 FLASH 碳离子的人体细胞实验。
开发了用于 FLASH 碳离子照射（1-3 Gy，13 或 50 keV/μm）的系统。通过结晶紫染色试验和衰老相关β-半乳糖苷酶染色分别评估 HFL1 肺成纤维细胞的生长和衰老。通过集落形成试验评估 HSGc-C5 癌细胞的存活。
碳离子的剂量率范围为 96-195 Gy/s，符合 FLASH 的定义。对于 13 和 50 keV/μm 射线，在 HFL1 细胞的生长抑制和衰老以及 HSGc-C5 细胞的存活方面均未观察到 FLASH 保护效应。
我们成功地进行了首次使用 FLASH 碳离子的人体细胞实验。在所检查的条件下，未观察到 FLASH 效应。
