Huygens-Kamerlingh Onnes Laboratory, Leiden University, P.O. Box 9504, 2333 CA Leiden, The Netherlands.
J Chem Phys. 2022 Apr 28;156(16):160903. doi: 10.1063/5.0087003.
We review some of the progress of single-molecule optical experiments in the past 20 years and propose some perspectives for the coming years. We particularly focus on methodological advances in fluorescence, super-resolution, photothermal contrast, and interferometric scattering and briefly discuss a few of the applications. These advances have enabled the exploration of new emitters and quantum optics; the chemistry and biology of complex heterogeneous systems, nanoparticles, and plasmonics; and the detection and study of non-fluorescing and non-absorbing nano-objects. We conclude by proposing some ideas for future experiments. The field will move toward more and better signals of a broader variety of objects and toward a sharper view of the surprising complexity of the nanoscale world of single (bio-)molecules, nanoparticles, and their nano-environments.
我们回顾了过去 20 年中单分子光学实验的一些进展，并为未来几年提出了一些展望。我们特别关注荧光、超分辨、光热对比和干涉散射方面的方法学进展，并简要讨论了一些应用。这些进展使得探索新的发射器和量子光学、复杂异质体系、纳米粒子和等离子体的化学和生物学、以及非荧光和非吸收纳米物体的检测和研究成为可能。最后，我们提出了一些未来实验的想法。该领域将朝着更多更好的信号、更广泛的各种物体的信号发展，并对单（生物）分子、纳米粒子及其纳米环境的纳米尺度世界的惊人复杂性有更清晰的认识。
