College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China.
College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China.
Food Chem. 2022 Sep 30;389:133070. doi: 10.1016/j.foodchem.2022.133070. Epub 2022 Apr 25.
Cordyceps militaris is an edible fungus that is widely used as a functional food in many countries. In order to objectively evaluate its nutritional value, free and glycosidic cordycepins need to be analyzed. The cordycepin arabinoside molecule was recognized by the MS fragmentation rule, and both cordycepin and its arabinoside were quantitatively analyzed in the fruiting body and pupa of Cordyceps militaris by high-performance liquid chromatography with tandem mass spectrometric (HPLC-MS/MS). The method had good linear regression (R = 0.9999), with a detection limit of 0.021 ng/mL. The recovery range was 94.32-103.09% in the fruiting body and pupa. The content of cordycepin and its arabinoside showed an upward trend with growth, and the total contents reached the highest level at the mature stage (60-70th day) without mildew. This study provides a useful reference for the evaluation and application of Cordyceps militaris as a functional food resource.
蛹虫草是一种可食用的真菌，在许多国家被广泛用作功能性食品。为了客观评估其营养价值，需要对游离态和糖苷态虫草素进行分析。根据 MS 裂解规律，鉴定了虫草素阿拉伯糖苷分子，并采用高效液相色谱-串联质谱法（HPLC-MS/MS）对蛹虫草子实体和蛹中的虫草素及其阿拉伯糖苷进行了定量分析。该方法具有良好的线性回归（R = 0.9999），检测限为 0.021ng/mL。在子实体和蛹中，该方法的回收率范围为 94.32%-103.09%。虫草素及其阿拉伯糖苷的含量随生长呈上升趋势，总含量在未霉变的成熟阶段（第 60-70 天）达到最高水平。本研究为蛹虫草作为功能性食品资源的评价和应用提供了有益参考。
