Sidki A M, Al-Abdulla I H, Rowell F J
Clin Chem. 1987 Apr;33(4):463-7.
We developed and validated a magnetizable solid-phase fluoroimmunoassay for directly determining quinine in serum or urine. We prepared the immunogen by coupling quinine hemisuccinate to keyhole-limpet hemocyanine, using this to immunize three sheep, and coupling the antisera to magnetizable solid-phase particles to facilitate separation of bound antigen from interfering components of serum or urine. We tested the stability of two fluorescein-labeled derivatives of quinine--one prepared by direct conjugation of quinine to dichlorotriazenyl aminofluorescein, the other by conjugating quinine hemisuccinate to fluorescein thiocarbamyl ethylenediamine. The latter was unstable. Using the former label and an 30-min incubation, we saw no interference by quinidine (an enantiomer of quinine) or other antimalarial drugs at their therapeutic concentrations.
我们开发并验证了一种可磁化的固相荧光免疫分析法，用于直接测定血清或尿液中的奎宁。我们通过将半琥珀酸奎宁与匙孔血蓝蛋白偶联来制备免疫原，用其免疫三只绵羊，并将抗血清与可磁化的固相颗粒偶联，以促进结合抗原与血清或尿液中的干扰成分分离。我们测试了两种奎宁的荧光素标记衍生物的稳定性——一种是通过将奎宁直接与二氯三嗪基氨基荧光素偶联制备的，另一种是通过将半琥珀酸奎宁与荧光素硫代氨基甲酰乙二胺偶联制备的。后者不稳定。使用前一种标记物并孵育30分钟，我们发现奎尼丁（奎宁的对映体）或其他治疗浓度的抗疟药物没有干扰。
