Babalola C P, Bolaji O O, Dixon P A, Ogunbona F A
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife, Nigeria.
J Chromatogr. 1993 Jun 23;616(1):151-4. doi: 10.1016/0378-4347(93)80482-j.
A new simple, selective and reproducible high-performance liquid chromatographic method for the determination of quinine in plasma, saliva and urine is described. The ion-pair method was carried out on a reversed-phase C18 column, using perchlorate ion as the counter ion and ultraviolet detection at 254 nm. Quinine was well resolved from its major metabolite, 3-hydroxyquinine, and the internal standard, primaquine. The limit of detection was 10 ng/ml and the recovery was greater than 90% from the three biological fluids.
本文描述了一种用于测定血浆、唾液和尿液中奎宁的新型简单、选择性和可重现的高效液相色谱法。离子对法在反相C18柱上进行，使用高氯酸根离子作为反离子，并在254nm处进行紫外检测。奎宁与其主要代谢物3-羟基奎宁以及内标伯氨喹得到了很好的分离。检测限为10ng/ml，三种生物流体中的回收率均大于90%。
