Racek J, Musil J
Clin Chim Acta. 1987 Jan 30;162(2):129-39. doi: 10.1016/0009-8981(87)90444-x.
The preparation of a biosensor for lactate determination is described. The biosensor is based on an immobilized suspension of the aerobic yeast Hansenula anomala, containing flavocytochrome b2 in high activity. The conditions for yeast cultivation were optimized to gain a sufficiently high activity of this enzyme converting lactate in the cells. The properties of the biosensor are compared with those of a sensor based on immobilized enzyme flavocytochrome b2. The yeast lactate biosensor has a sufficient sensitivity and linearity and short time of response. The precision and accuracy of lactate determination as well as the results of comparisons using an enzyme electrode and the spectrophotometric UV-test, enables this biosensor to be used in routine work. Analysis can be performed in blood plasma or whole blood. The stability of the biosensor makes it possible to work for 4 weeks with one yeast cell pellet.
本文描述了一种用于测定乳酸的生物传感器的制备方法。该生物传感器基于固定化的需氧酵母异常汉逊酵母悬浮液，其含有高活性的黄素细胞色素b2。优化了酵母培养条件，以获得该酶在细胞中转化乳酸的足够高的活性。将该生物传感器的性能与基于固定化酶黄素细胞色素b2的传感器的性能进行了比较。酵母乳酸生物传感器具有足够的灵敏度、线性度和较短的响应时间。乳酸测定的精密度和准确度以及使用酶电极和分光光度紫外测试的比较结果，使得该生物传感器能够用于常规工作。分析可以在血浆或全血中进行。该生物传感器的稳定性使得一个酵母细胞沉淀能够工作4周。
