Leung D Y, Schneeberger E E, Siraganian R P, Geha R S, Bhan A K
Clin Immunol Immunopathol. 1987 Mar;42(3):328-37. doi: 10.1016/0090-1229(87)90021-3.
Monoclonal antibodies reactive with human IgE were used to investigate the presence of surface IgE in situ on mononuclear cells infiltrating into the skin lesion of atopic dermatitis (A.D.) by application of the immunoperoxidase technique to tissue sections for light and electron microscopic examination. A substantial proportion of infiltrating macrophages but not of lymphocytes were found to bear IgE on their cell surfaces. These observations raise the possibility that IgE may contribute to the skin inflammation associated with A.D. via non-mast-cell-mediated immune mechanisms. We hypothesize that allergens introduced into the skin lesion of A.D. are potentially capable of interacting not only with IgE-bearing mast cells but also with IgE-bearing macrophages and dendritic cells to cause the release of inflammatory mediators.
使用与人IgE反应的单克隆抗体，通过将免疫过氧化物酶技术应用于组织切片进行光镜和电镜检查，来研究浸润到特应性皮炎（A.D.）皮肤病变中的单核细胞原位表面IgE的存在情况。发现相当一部分浸润的巨噬细胞而非淋巴细胞在其细胞表面带有IgE。这些观察结果提出了一种可能性，即IgE可能通过非肥大细胞介导的免疫机制促成与A.D.相关的皮肤炎症。我们推测，引入A.D.皮肤病变中的变应原不仅有可能与带有IgE的肥大细胞相互作用，还能与带有IgE的巨噬细胞和树突状细胞相互作用，从而导致炎症介质的释放。
