Federal University of Paraná, Pharmacology Department, Curitiba, Brazil.
Federal University of Santa Catarina, Laboratory of Neurobiology of Pain and Inflammation, Physiological Sciences Department, Santa Catarina, Brazil.
Behav Brain Res. 2022 Jul 5;429:113905. doi: 10.1016/j.bbr.2022.113905. Epub 2022 Apr 28.
The flavonoid myricitrin showed an antidepressant-like effect in the tail suspension test and increased hippocampal neurogenesis, as well as demonstrating anti-inflammatory effects. Interestingly, inflammation has been linked to depression, and anti-inflammatory drugs showed promising results as antidepressant-like drugs. Thus, the present study evaluated the effects of myricitrin in the chronic mild stress (CMS) model, a translational and valid animal model of depression, using the mini-experiment design to improve the reproducibility of the findings. The sucrose preference test (SPT), forced swim test (FST), and tail suspension test (TST) were the readouts of depressive-like phenotypes induced by CMS. Relative adrenal weight was employed as an index of the hypothalamus-pituitary-adrenal (HPA) axis activation. Interleukin (IL)-6 and tumor necrosis factor (TNF)-alpha levels were measured in the hippocampus. Myricitrin (10 mg/kg, intraperitoneally, for 14 days) reversed depressive-like behaviors induced by CMS (increased immobility in the FST, the TST and anhedonia), as well as decreased adrenal hypertrophy and hippocampal levels of IL-6 in stressed mice. Similar results were observed by imipramine (20 mg/kg, intraperitoneally, for 14 days), a serotonin and norepinephrine reuptake inhibitor (positive control). A significant correlation was observed between immobility time in the TST, and hippocampal IL-6 levels. Hippocampal TNF-α levels were not affected by CMS or drug treatment. In conclusion, myricitrin exhibited an antidepressant-like profile in CMS, and this effect may be associated with its anti-inflammatory activity.
杨梅素具有抗抑郁样作用，可增加海马神经发生，并具有抗炎作用。有趣的是，炎症与抑郁症有关，抗炎药物作为抗抑郁样药物显示出良好的效果。因此，本研究采用迷你实验设计，评估了杨梅素在慢性轻度应激（CMS）模型中的作用，CMS 是一种具有转化和有效性的抑郁症动物模型，以提高研究结果的可重复性。糖水偏好试验（SPT）、强迫游泳试验（FST）和悬尾试验（TST）是 CMS 诱导的抑郁样表型的检测指标。相对肾上腺重量被用作下丘脑-垂体-肾上腺（HPA）轴激活的指标。在海马体中测量白细胞介素（IL）-6 和肿瘤坏死因子（TNF）-α 水平。杨梅素（10mg/kg，腹腔注射，14 天）逆转了 CMS 诱导的抑郁样行为（增加 FST、TST 中的不动时间和快感缺失），并降低了应激小鼠的肾上腺肥大和海马体中 IL-6 的水平。三环类抗抑郁药（20mg/kg，腹腔注射，14 天），一种 5-羟色胺和去甲肾上腺素再摄取抑制剂（阳性对照）也观察到了类似的结果。TST 中的不动时间与海马体 IL-6 水平之间存在显著相关性。CMS 或药物处理对海马体 TNF-α 水平没有影响。总之，杨梅素在 CMS 中表现出抗抑郁样作用，这种作用可能与其抗炎活性有关。
