Department of Physiology, Zanjan Branch, Islamic Azad University, Zanjan, Iran.
Division of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, 14155-6453 Tehran, Iran.
Peptides. 2022 Jul;153:170803. doi: 10.1016/j.peptides.2022.170803. Epub 2022 Apr 28.
There are some differences between mammals and birds in terms of central food intake regulation. In avian species, the hypophagic role of nesfatin-1 has not been investigated with other neurotransmitters. Therefore, this study aimed to determine the alteration of feeding behavior following intracerebroventricular (ICV) injection of nesfatin-1 and its possible interaction with central noradrenergic, serotoninergic, and oxytocin systems in newborn broiler chicks. In experiment 1, birds received ICV injection of phosphate-buffered saline (PBS), prazosin (α receptors antagonist, 10 nmol), nesfatin-1 (40 ng), and co-administration of prazosin and nesfatin-1. Experiments 2-10 were similar to experiment 1, except that yohimbine (α receptors antagonist, 13 nmol), metoprolol (β receptors antagonist, 24 nmol), IC1118,551 (β receptors antagonist for, 5nmol), SR59230R (β receptors antagonist, 20 nmol), fluoxetine (serotonin reuptake inhibitor, 10 µg), PCPA (serotonin synthesis inhibitor, 1.5 µg), 8-OH-DPAT (5-HTA receptors agonist, 15.25 nmol), SB242084 (5-HTC receptors antagonist,1.5 µg) and tocinoic acid (oxytocin receptors antagonist, 2 µg) were injected instead of prazosin. Immediately after the injection, food consumption and behavioral traits were recorded. Nesfatin-1 decreased food consumption (P < 0.05). Nesfatin-1 along with ICI118551 decreased food consumption (P < 0.05). The nesfatin-1- induced hypophagia were reduced by the simultaneous injection of PCPA and nesfatin-1 (P < 0.05). Nesfatin-1induced hypophagia were decreased by the simultaneous injection of SB242084 (P < 0.05). The nesfatin-1 -induced hypophagia were abolished by the simultaneous injection of the tocinoic acid and nesfatin-1 (P < 0.05). ICV injection of the nesfatin-1 decreased the number of steps, jumps, exploratory food, and pecks (P < 0.05) with no effect on drink pecks (P > 0.05). Nesfatin-1 significantly decreased standing time and increased both sitting time and rest time (P < 0.05). Nesfatin-1 could play an important role in feeding behavior, and its hypophagic effects were mediated by β adrenergic, 5-HTC serotoninergic, and oxytocin receptors in neonatal chickens.
哺乳动物和鸟类在中枢进食调节方面存在一些差异。在鸟类中，nesfatin-1 的抑食作用尚未与其他神经递质一起进行研究。因此，本研究旨在确定新生肉鸡雏鸡脑室内（ICV）注射 nesfatin-1 后摄食行为的改变及其与中枢去甲肾上腺素能、5-羟色胺能和催产素系统的可能相互作用。在实验 1 中，鸟类接受 ICV 注射磷酸盐缓冲盐水（PBS）、哌唑嗪（α 受体拮抗剂，10 nmol）、nesfatin-1（40 ng）和哌唑嗪与 nesfatin-1 共同给药。实验 2-10 与实验 1 相似，除了育亨宾（α 受体拮抗剂，13 nmol）、美托洛尔（β 受体拮抗剂，24 nmol）、IC1118551（β 受体拮抗剂，5 nmol）、SR59230R（β 受体拮抗剂，20 nmol）、氟西汀（5-羟色胺再摄取抑制剂，10 μg）、PCPA（5-羟色胺合成抑制剂，1.5 μg）、8-OH-DPAT（5-HTA 受体激动剂，15.25 nmol）、SB242084（5-HTC 受体拮抗剂，1.5 μg）和托西诺酸（催产素受体拮抗剂，2 μg）替代哌唑嗪。注射后立即记录食物消耗和行为特征。nesfatin-1 减少食物消耗（P<0.05）。nesfatin-1 与 ICI118551 减少食物消耗（P<0.05）。同时注射 PCPA 和 nesfatin-1 可减少 nesfatin-1 诱导的食欲减退（P<0.05）。同时注射 SB242084 可减少 nesfatin-1 诱导的食欲减退（P<0.05）。同时注射托西诺酸和 nesfatin-1 可消除 nesfatin-1 诱导的食欲减退（P<0.05）。ICV 注射 nesfatin-1 减少了步数、跳跃、探索性进食和啄食次数（P<0.05），但对饮水啄食次数没有影响（P>0.05）。nesfatin-1 显著减少站立时间，增加坐立时间和休息时间（P<0.05）。nesfatin-1 可能在摄食行为中发挥重要作用，其抑食作用是通过新生鸡的β肾上腺素能、5-HTC 5-羟色胺能和催产素受体介导的。
