Heidarzadeh Hooman, Zendehdel Morteza, Babapour Vahab, Gilanpour Hasan
Department of Basic Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, 14155-6453, Tehran, Iran.
Vet Res Commun. 2018 Mar;42(1):39-47. doi: 10.1007/s11259-017-9706-9. Epub 2017 Dec 26.
The present study was designed to determine the effect of central injection of Nesfatin-1 and corticotropin and histaminergic systems on food intake in neonatal meat-type chicks. In this study, 7 experiments were designed, each with 4 treatment groups. In experiment 1, four groups of chicks received the ICV injection of (A) phosphate-buffered saline (PBS), (B) Nesfatin-1 (10 ng), (C) Nesfatin-1 (20 ng) and (D) Nesfatin-1 (40 ng). In experiment 2, (A) PBS, (B) Astressin-B (CRF/CRF receptors antagonist; 30 µg), (C) Nesfatin-1 (40 ng) and (D) Nesfatin-1 + Astressin-B were injected. In experiments 3-6, chicken received ICV injection of the Astressin2-B (CRF receptor antagonist; 30 µg), α-FMH (alpha fluoromethyl histidine; as inhibitor of histidine decarboxylase, 250 nmol), Chlorpheniramine (histamine H receptors antagonist, 300 nmol), Famotidine (histamine H receptors antagonist, 82 nmol) and Thioperamide (histamine H receptors antagonist, 300 nmol) instead of the Astressin-B. Then the cumulative food intake measured until 120 min post-injection. According to the results, ICV injection of Nesfatin-1 dose dependently decreased food intake in neonatal chicks (P < 0.05). Co-injection of the Nesfatin-1 and Astressin-B (CRF/CRF) inhibited Nesfatin-1 induced hypophagia (P < 0.05). ICV inejction of the Nesfatin-1 + Astressin-B significantly inhibited the effect of Nesfatin-1 on food intake (P < 0.05). In addition, α-FMH and chlorpheniramine attenuated Nesfatin-1-induced hypophagia in chicks (P < 0.05); while thioperamide significantly amplified the effect of Nesfatin-1 on food intake in chicks (P < 0.05). These results suggested Nesfatin-1 has an anorectic effect in 3-hour food deprived neonatal meat-type chicks and this effect was mediated by corticotropin CRF/CRF as well as histamine H and H receptors.
本研究旨在确定向新生肉用型雏鸡中枢注射Nesfatin-1、促肾上腺皮质激素和组胺能系统对其采食量的影响。本研究设计了7个实验，每个实验有4个处理组。在实验1中，四组雏鸡接受脑室内注射：(A) 磷酸盐缓冲盐水（PBS）、(B) Nesfatin-1（10纳克）、(C) Nesfatin-1（20纳克）和(D) Nesfatin-1（40纳克）。在实验2中，注射(A) PBS、(B) 阿斯特辛-B（促肾上腺皮质激素释放因子/促肾上腺皮质激素释放因子受体拮抗剂；30微克）、(C) Nesfatin-1（40纳克）和(D) Nesfatin-1 + 阿斯特辛-B。在实验3 - 6中，雏鸡接受脑室内注射阿斯特辛2-B（促肾上腺皮质激素释放因子受体拮抗剂；30微克）、α-氟甲基组氨酸（作为组氨酸脱羧酶抑制剂，250纳摩尔）、氯苯那敏（组胺H受体拮抗剂，300纳摩尔）、法莫替丁（组胺H受体拮抗剂，82纳摩尔）和硫代乙酰胺（组胺H受体拮抗剂，300纳摩尔），以替代阿斯特辛-B。然后测量注射后120分钟内的累积采食量。结果显示，脑室内注射Nesfatin-1可使新生雏鸡的采食量呈剂量依赖性下降（P < 0.05）。Nesfatin-1与阿斯特辛-B（促肾上腺皮质激素释放因子/促肾上腺皮质激素释放因子）共同注射可抑制Nesfatin-1诱导的摄食减少（P < 0.05）。脑室内注射Nesfatin-1 + 阿斯特辛-B可显著抑制Nesfatin-1对采食量的影响（P < 0.05）。此外，α-氟甲基组氨酸和氯苯那敏可减弱Nesfatin-1诱导的雏鸡摄食减少（P < 0.05）；而硫代乙酰胺可显著增强Nesfatin-1对雏鸡采食量的影响（P < 0.05）。这些结果表明，Nesfatin-1对禁食3小时的新生肉用型雏鸡有厌食作用，且这种作用由促肾上腺皮质激素促肾上腺皮质激素释放因子/促肾上腺皮质激素释放因子以及组胺H和H受体介导。
