Daneshvar M, Zendehdel M, Vazir B, Asghari A
Department of Basic Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Arch Razi Inst. 2022 Feb 28;77(1):141-149. doi: 10.22092/ARI.2021.354450.1638. eCollection 2022 Feb.
The current study was conducted to investigate the interaction between the central adrenergic and histaminergic systems and the broiler chick's feed intake. In the first experiment, the intracerebroventricular (ICV) injection of solutions was conducted which included 10 nmol of prazosin (an α-receptor antagonist), 300 nmol of histamine, co-injection of prazosin and histamine. Experiments two to five were conducted similarly the same as the first experiment, in which chickens were ICV injected with 13 nmol of yohimbine (an α-receptor antagonist), 24 nmol of metoprolol (a β adrenergic receptor antagonist), 5 nmol of ICI 118,551 (a β adrenergic receptor antagonist), and 20 nmol of SR 59230R (a β adrenergic receptor antagonist). The injected solutions in the sixth experiment included 300 nmol of noradrenaline, 250 nmol of α-FMH (an alpha fluoromethyl histidine), noradrenaline, and α-FMH. Seventh to ninth experiments were similar to the sixth experiment, except that the chickens were ICV injected with 300 nmol of chlorpheniramine (a histamine H receptors antagonist), 82 nmol of famotidine (a histamine H receptors antagonist), and 300 nmol of thioperamide (a histamine H receptors antagonist), rather than α-FMH. Afterward, the cumulative food intake was measured 120 min after injection. Based on the obtained results, both histamine ICV injection and noradrenaline injection reduced food intake (<0.05). Moreover, co-injection of histamine and ICI 118,551 (<0.05), and co-injection of noradrenaline and Chlorpheniramine reduced food intake (<0.05). In addition, noradrenaline and Thioperamide co-injection improved hypophagic effect of noradrenaline in neonatal chicken (<0.05). These findings suggested the effect of interconnection between adrenergic and histaminergic systems, which may be mediated by H and H histaminergic and β adrenergic receptors, on the regulation of food intake in the neonatal broiler chicken.
本研究旨在探讨中枢肾上腺素能系统和组胺能系统之间的相互作用以及对肉鸡采食量的影响。在第一个实验中，通过脑室内（ICV）注射溶液，其中包括10 nmol的哌唑嗪（一种α受体拮抗剂）、300 nmol的组胺、哌唑嗪和组胺的联合注射。实验二至五与第一个实验类似，鸡通过ICV注射13 nmol的育亨宾（一种α受体拮抗剂）、24 nmol的美托洛尔（一种β肾上腺素能受体拮抗剂）、5 nmol的ICI 118,551（一种β肾上腺素能受体拮抗剂）和20 nmol的SR 59230R（一种β肾上腺素能受体拮抗剂）。第六个实验中的注射溶液包括300 nmol的去甲肾上腺素、250 nmol的α-氟甲基组氨酸（α-FMH）、去甲肾上腺素和α-FMH。第七至九个实验与第六个实验相似，不同之处在于鸡通过ICV注射300 nmol的氯苯那敏（一种组胺H受体拮抗剂）、82 nmol的法莫替丁（一种组胺H受体拮抗剂）和300 nmol的硫代哌酰胺（一种组胺H受体拮抗剂），而不是α-FMH。之后，在注射后120分钟测量累积采食量。根据所得结果，脑室内注射组胺和去甲肾上腺素均降低了采食量（<0.05）。此外，组胺与ICI 118,551的联合注射（<0.05）以及去甲肾上腺素与氯苯那敏的联合注射降低了采食量（<0.05）。此外，去甲肾上腺素和硫代哌酰胺的联合注射增强了去甲肾上腺素对新生鸡的厌食作用（<0.05）。这些发现表明肾上腺素能系统和组胺能系统之间的相互联系，可能由H和H组胺能受体以及β肾上腺素能受体介导，对新生肉鸡采食量的调节产生影响。
