Guo Xiaohu, Meng Xiaoxi, Li Yan, Qu Changqing, Liu Yingying, Cao Mengyang, Yao Xiaoyan, Meng Fei, Wu Jing, Peng Huasheng, Peng Daiyin, Xing Shihai, Jiang Weimin
College of Pharmacy, Anhui University of Chinese Medicine, Hefei, 230012, China.
Department of Horticultural Science, University of Minnesota, MN, 55108, USA.
J Ethnopharmacol. 2022 Jul 15;293:115329. doi: 10.1016/j.jep.2022.115329. Epub 2022 Apr 28.
Salvia miltiorrhiza Bunge is a bulk medicinal material used in traditional Chinese medicine, that can cure cardiovascular diseases, neurasthenia, and other conditions. Sweating is a frequently used method of processing S. miltiorrhiza for medical applications. We previously demonstrated changes to the metabolic profile of linoleic acid, glyoxylate, and dicarboxylate after Sweating. However, this alteration has not been explained at the molecular level.
Fresh roots of Salvia miltiorrhiza Bunge were treated by the Sweating processing, and then the tandem mass tag technique was used to compare the proteome difference between Sweating S. miltiorrhiza and non-Sweating S. miltiorrhiza.
We identified a total of 850 differentially expressed proteins after Sweating treatment in S. miltiorrhiza, including 529 upregulated proteins and 321 downregulated proteins. GO enrichment analysis indicated that these differentially expressed proteins are involved in external encapsulating structure, cell wall, oxidoreductase activity, ligase activity, and others. Further analysis showed that CYP450, the pathogenesis-related protein Bet v 1 family, and the peroxidase domain were the major protein domains. KEGG enrichment identified 18 pathways, of which phenylpropanoid biosynthesis is the most important one related to the metabolite profile and is the principal chemical component of S. miltiorrhiza.
This study addressed potential molecular mechanisms in S. miltiorrhiza after Sweating, and the findings provide reasons for the changes in biochemical properties and metabolites changes which might cause pharmacological variation at the proteome level.
丹参是一种大宗中药材，可治疗心血管疾病、神经衰弱等病症。发汗是丹参入药常用的炮制方法。我们之前证明了发汗后亚油酸、乙醛酸和二羧酸的代谢谱发生了变化。然而，这种变化尚未在分子水平上得到解释。
将新鲜丹参根进行发汗处理，然后采用串联质量标签技术比较发汗丹参与未发汗丹参的蛋白质组差异。
我们共鉴定出发汗处理后丹参中有850个差异表达蛋白，其中上调蛋白529个，下调蛋白321个。基因本体富集分析表明，这些差异表达蛋白涉及外部包被结构、细胞壁、氧化还原酶活性、连接酶活性等。进一步分析表明，细胞色素P450、病程相关蛋白Bet v 1家族和过氧化物酶结构域是主要的蛋白结构域。京都基因与基因组百科全书富集分析确定了18条通路，其中苯丙烷生物合成是与代谢物谱最相关的一条通路，也是丹参的主要化学成分。
本研究探讨了丹参发汗后的潜在分子机制，研究结果为生化性质变化和代谢物变化提供了原因，这些变化可能在蛋白质组水平上导致药理变异。
