Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 40402, Taiwan.
Institute of Plant and Microbial Biology, Academia Sinica, Taipei 11529, Taiwan.
J Photochem Photobiol B. 2018 Jun;183:164-171. doi: 10.1016/j.jphotobiol.2018.04.013. Epub 2018 Apr 11.
The effect of light-emitting diodes (LEDs) on the production of secondary metabolites in medicinal plants and hairy roots is receiving much attention. The roots and rhizomes of the traditional Chinese medicinal plant Salvia miltiorrhiza Bunge are widely used for treating cardiovascular and cerebrovascular diseases. The main components are liposoluble tanshinones and hydrophilic phenolic acids. Moreover, hairy root culture of S. miltiorrhiza has been used in research of valuable plant-derived secondary metabolites. In this study, we examined the effect of LEDs with different combinations of wavelengths on the content of the main components in hairy roots of S. miltiorrhiza. Tanshinone IIA (TSIIA) content in hairy roots was significantly decreased with all light treatments containing blue light by >60% and was 9 times lower with LED treatment duration changed from 1 week to 3 weeks. HMGR, DXS2, DXR, GGPPS, CPS and CYP76AH1 genes involved in the tanshinone biosynthesis pathway were downregulated by blue light. Furthermore, light quality treatments have different effect on the accumulation of phenolic acids in hairy roots of S. miltiorrhiza. The light treatments 6R3B, 6B3IR, 7RGB and 2R6BUV for 3 weeks could increase rosmarinic acid (RA) content slightly but not salvianolic acid B (SAB) content. Different secondary metabolite contents could be regulated by different wavelength combinations of LEDs. Blue light could reduce TSIIA content in hairy roots of S. miltiorrhiza via gene regulation.
发光二极管（LED）对药用植物和毛状根次生代谢产物的产生的影响正受到广泛关注。传统中药丹参的根和根茎广泛用于治疗心脑血管疾病。主要成分是脂溶性丹参酮和水溶性酚酸。此外，丹参毛状根培养已用于研究有价值的植物衍生次生代谢产物。在这项研究中，我们研究了不同波长组合的 LED 对丹参毛状根主要成分含量的影响。含有蓝光的所有光照处理均使毛状根中的丹参酮 IIA（TSIIA）含量显著降低>60%，而 LED 处理时间从 1 周变为 3 周时，TSIIA 含量降低了 9 倍。参与丹参酮生物合成途径的 HMGR、DXS2、DXR、GGPPS、CPS 和 CYP76AH1 基因被蓝光下调。此外，不同的光质处理对丹参毛状根中酚酸的积累有不同的影响。3 周的 6R3B、6B3IR、7RGB 和 2R6BUV 光照处理可略微增加迷迭香酸（RA）含量，但不增加丹酚酸 B（SAB）含量。不同的次生代谢产物含量可以通过不同波长组合的 LED 进行调节。蓝光可以通过基因调控降低丹参毛状根中 TSIIA 的含量。
